番茄红素基因工程菌多酶调控研究进展

发布时间：2018-05-11 11:52

本文选题：番茄红素 + 多酶调控　；参考：《生物工程学报》2017年04期

【摘要】：作为类异戊二烯化合物中四萜的代表性产品番茄红素,在生物体中是典型的多酶参与催化的反应产物,在2-甲基-D-赤藓糖醇-4-磷酸盐(MEP)和甲羟戊酸(MVA)合成途径中起着至关重要的作用。从番茄红素在原核和真核中的多酶合成途径出发,针对番茄红素合成途径的各种优化策略,首先介绍了多酶合成中的常规调控方法,包括多基因共表达质粒构建、基因顺序调控、启动子与核糖体结合位点调控及基因敲除和替换等方法。之后介绍了一些新型的多酶调控方法,包括多片段组装技术、人工支架自组装方法等。最后重点介绍了这些多酶调控方法在番茄红素合成中的应用。这些多酶合成调控方法为构建高产番茄红素菌株提供了极大的启发和研究基础。
[Abstract]:As the representative product of tetraterpenes in isoprene compounds, lycopene is a typical multi-enzyme reaction product in organism. It plays an important role in the synthesis of 2-methyl-Derythritol -4-phosphate (MEP) and mevalerate (MVA). Based on the multi-enzyme synthesis pathway of lycopene in prokaryotic and eukaryotic systems, the conventional regulation methods of lycopene biosynthesis, including the construction of multi-gene coexpression plasmid, were introduced in allusion to various optimization strategies of lycopene biosynthesis pathway. Gene sequence regulation, promoter-ribosomal binding site regulation, gene knockout and substitution. Then some new multi-enzyme regulation methods are introduced, including multi-fragment assembly, self-assembly of artificial scaffolds and so on. Finally, the application of these methods in lycopene synthesis was introduced. These methods provide great inspiration and research basis for the construction of lycopene-producing strains.
【作者单位】：南京工业大学生物与制药工程学院材料化学工程国家重点实验室;扬州工业职业技术学院化学工程学院;南京工业大学食品与轻工学院材料化学工程国家重点实验室;南京工业大学药学院;
【基金】：国家自然科学基金(No.21406111) 江苏省自然科学基金(No.BK20130917) 大学生创新与实验开放基金(No.2016DC365)资助~~
【分类号】：O629;Q78

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