天然产物来源多糖结构解析研究进展

发布时间：2018-05-11 13:05

  本文选题：多糖 + 结构　； 参考：《中国食品学报》2017年03期

【摘要】：多糖是高等植物、动物、微生物细胞中一类非常重要的天然大分子物质,由于许多活性多糖具有良好的免疫调节、抗肿瘤、抗氧化和调节肠道菌群等功能,因此科学认识多糖结构有利于深入研究其生物活性功能,对多糖的基础研究和应用具有重要意义。本文简述天然产物来源多糖分离纯化和结构解析的研究方法,重点介绍葡聚糖、葡甘聚糖、木葡聚糖、阿拉伯木聚糖和果胶等多糖结构解析的研究进展,就多糖结构解析目前存在的一些问题进行探讨与展望。通过归纳文献资料并结合笔者团队近20年的研究积累,提出"部分降解-甲基化-核磁共振"可作为多糖一级结构解析的核心技术,同时可辅助利用ESI-MS、Maldi-Tof-MS、离子淌度-质谱联用等技术与方法,从而构建一套先进的多糖一级结构解析技术体系。
[Abstract]:Polysaccharide is a kind of very important natural macromolecular substance in higher plants, animals and microorganism cells, because many active polysaccharides have good functions of immune regulation, anti-tumor, anti-oxidation and regulation of intestinal flora, etc. Therefore, scientific understanding of the structure of polysaccharides is beneficial to the further study of their biological activity and function, and is of great significance to the basic research and application of polysaccharides. In this paper, the methods of isolation, purification and structure analysis of polysaccharides from natural products are reviewed, and the progress in structural analysis of polysaccharides, such as dextran, glucomannan, xyloglucan, arabinoxylan and pectin, is emphasized. Some problems existing in the structure analysis of polysaccharides were discussed and prospected. By summarizing the literature and combining the research and accumulation of the author's team in the past 20 years, it is proposed that "partial degradation-methylation-nuclear magnetic resonance" can be used as the core technology for the analysis of the primary structure of polysaccharides. At the same time, we can use ESI-MSN Maldi-Tof-MSand ion mobility mass spectrometry to construct a set of advanced primary structure analysis technology system of polysaccharides.
【作者单位】： 南昌大学食品科学与技术国家重点实验室;
【基金】：国家“863”计划项目(2013AA102102) 国家自然科学基金项目(31571826,21564007)
【分类号】：O629.12
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本文编号：1874044