基于UPLC-Q-TOF MS技术的三七中皂苷类成分质谱裂解规律研究

发布时间：2018-05-13 18:03

本文选题：超高效液相色谱-串联四极杆飞行时间质谱(UPLC-Q-TOF + MS)　；参考：《质谱学报》2017年01期

【摘要】：采用超高效液相色谱-串联四极杆飞行时间质谱(UPLC-Q-TOF MS)法快速分析三七中17种化合物,包括4对人参皂苷同分异构体,即三七皂苷R_1、R_2,人参皂苷Rg_1,人参皂苷Rg_3、Rb_1、Rb_2、Rb_3、Re、Rf、Rc、Ro、Rd、Rk_1、Rh_1和拟人参皂苷_(F11)。采用Waters Acquity UPLC~(TM)BEH C18色谱柱(2.1mm×150mm×1.7μm),以乙腈(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱,在电喷雾负离子模式下采集数据。结果表明,17种常见的三七皂苷和人参皂苷化合物对照品(包含多种同分异构体皂苷)可被液相色谱完全分离,通过归纳总结质谱全裂解信息,探讨了其裂解规律和特征离子。该方法可为快速鉴定和分析含有三七皂苷和人参皂苷类成分的化合物提供参考,并为全面表征三七指纹图谱提供依据。
[Abstract]:The rapid analysis of 17 compounds in Panax notoginseng was carried out by UPLC-Q-TOF MS, including four pairs of ginsenoside isomers, namely, Panax notoginsenoside R1C R2, Panax notoginsenoside Rg1, Rg1Rb2Rb2Rb3Rb3Rb3Rb3Rb3Rb3Rb3ReRfRfRfRfRfRb3ReRfRfRfRfRfrcrcrcrkkkkkk1rr1 and Panax saponins R1R1R1, Rg3Rb1Rb2Rb2Rb2Rb3Rb3Rb3Retf RfRfRfRfRfrcrc@@ A Waters Acquity UPLC~(TM)BEH C18 column of 2.1 mm 脳 150mm 脳 1.7 渭 m was used for gradient elution with acetonitrile (acetonitrile) -0.1% formic acid aqueous solution as mobile phase, and the data were collected by electrospray anion mode. The results showed that 17 common reference compounds of Panax notoginseng saponins and ginsenoside compounds (including many isomers saponins) could be completely separated by liquid chromatography. By summing up the information of total cracking of mass spectrometry, the cracking law and characteristic ions were discussed. The method can provide a reference for rapid identification and analysis of compounds containing panax notoginsenosides and ginsenosides, and provide a basis for the comprehensive characterization of Panax notoginseng fingerprints.
【作者单位】：北京中医药大学;国家知识产权局专利局专利审查协作四川中心;首都医科大学附属北京中医医院北京市中医研究所;
【分类号】：R284.1;O657.63

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