在线固相萃取-高效液相色谱检测饮料中5种食用合成色素

发布时间：2018-05-13 17:57

本文选题：在线固相萃取 + 食用合成色素　；参考：《分析测试学报》2016年12期

【摘要】：建立了同时测定饮料中5种食用合成色素的在线固相萃取-高效液相色谱-紫外检测(Online-SPE-HPLC-UV)方法。饮料样品用水稀释并经0.45μm微孔滤膜过滤后,在线固相萃取柱(Acclaim 120 C18)净化,反相C18分析柱分离,梯度洗脱,紫外检测器检测。结果表明:5种食用合成色素在0.5~20 mg/L浓度范围内线性关系良好,相关系数(r2)均大于0.999 9,检出限为0.02~0.08 mg/kg,3个水平下的加标回收率为94.5%~103.0%,相对标准偏差为0.4%~2.7%。该研究为快速准确地分离测定饮料中的食用合成色素提供了有效方法。
[Abstract]:An on-line SPE-SPE-HPLC-UV method for simultaneous determination of five food synthetic pigments in beverages was developed. The beverage samples were diluted with water and filtered by 0.45 渭 m microporous membrane, then purified by on-line solid phase extraction (SPE) column acclaim 120 C18), separated by reversed phase C 18 column, gradient eluted and detected by UV detector. The results showed that there was a good linearity in the concentration range of 0.5 ~ 20 mg/L for 5 kinds of food synthetic pigments. The correlation coefficient (r _ 2) was more than 0.999, the detection limit was 0.02 ~ 0.08 mg / kg, the recovery rate was 94.55 ~ 103.0% and the relative standard deviation was 0.4 ~ (2. 7) 路kg ~ (-1). This study provides an effective method for the rapid and accurate separation and determination of edible synthetic pigments in beverages.
【作者单位】：东南大学公共卫生学院;江苏省疾病预防控制中心;
【基金】：江苏省科技厅科技支撑计划项目(BE2010745)
【分类号】：O657.72;TS27

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