化妆品中米诺地尔的毛细管区带电泳测定法

发布时间：2018-05-13 19:29

本文选题：米诺地尔 + 毛细管区带电泳　；参考：《环境与健康杂志》2017年08期

【摘要】：目的建立化妆品中米诺地尔的毛细管区带电泳(CZE)测定法。方法样品经提取液(甲醇∶2 mmol/L磷酸二氢钠+3 mmol/L磷酸体积比为1∶9)提取后离心,过0.45μm膜后上机。采用未涂敷熔融石英毛细管[75μm×30.2 cm(有效长度:20 cm)];分离缓冲液为20 mmol/L磷酸二氢钠-30 mmol/L磷酸,pH 2.13;分离电压为11 kV;检测波长为278 nm;进样压力为0.5 psi;进样时间为3 s。结果在1~160 mg/L的线性范围内,所得米诺地尔的回归方程为y=151.12x+73.363,r=0.999 95,呈良好的线性关系。该方法的检出限为0.3 mg/L,定量下限为1.0 mg/L,平均回收率在93.4%~95.9%之间,RSD在3.3%~3.7%之间。结论该方法简单、快速、准确,可用于化妆品中米诺地尔含量的检测。
[Abstract]:Objective to establish a capillary zone electrophoresis (CZE) method for the determination of minoxidil in cosmetics. Methods the sample was extracted from the extract solution (methanol: 2 mmol/L sodium dihydrogen phosphate 3 mmol/L phosphoric acid volume ratio 1: 9) and centrifuged. The uncoated fused quartz capillary [75 渭 m 脳 30.2 cm (effective length: 20 cm)] was used, the separation buffer was 20 mmol/L sodium dihydrogen phosphate -30 mmol/L phosphate pH 2.13, the separation voltage was 11 KV, the detection wavelength was 278nm, the injection pressure was 0.5 psii and the injection time was 3 s. Results within the linear range of 1: 160 mg/L, the regression equation of minodil was YJ 151.12x 73.363C 0.999 95, showing a good linear relationship. The detection limit of this method is 0.3 mg / L, the limit of quantification is 1.0 mg / L, and the average recovery is between 93.44% and 95.9% (RSD = 3.3%). Conclusion the method is simple, rapid and accurate, and can be used for the determination of minoxidil in cosmetics.
【作者单位】：北京市疾病预防控制中心中心实验室食物中毒诊断溯源技术北京市重点实验室;北京市预防医学研究中心;北京化工大学理学院;首都医科大学公共卫生学院;
【基金】：北京市卫生系统高层次卫生技术人才培养项目(2013-2-036)
【分类号】：O658.9;TQ658

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