手性和非手性偏振和频光谱技术在生物膜界面动力学研究中的应用

发布时间：2018-05-25 00:04

本文选题：和频光谱 + 手性　；参考：《中国科学技术大学》2017年硕士论文

【摘要】：在生命体内,特定手性的分子才能发挥特定的功能,以维持正常的生命活动。研究生物分子在生物膜界面的手性问题对于正确理解某些疾病的发病机理非常重要。因此加深对生物膜界面的手性生物分子的研究有助于理解相关疾病的发生机理,也为新型药物的研究、合成提供指导。然而生物膜界面的手性分子的研究很复杂,目前有效、实时、原位、免标记表征的研究手段很少。针对这一现状,本文利用界面敏感的、手性和非手性偏振结合的和频光谱测量技术,以两种手性生物分子,即胆固醇和hIAPP蛋白为模型,系统研究其在生物膜界面的组装和运输行为,以及蛋白在生物膜界面的结构转变过程。通过研究胆固醇分子在电中性的PC类磷脂膜和负电荷PG类磷脂膜中的翻转运输行为以及组装方式,给出胆固醇分子在此体系中的翻转运输时间尺度以及组装方式,得出胆固醇在磷脂双层膜中是否翻转主要取决于磷脂是否翻转的结论。同时在PG类磷脂膜中引入盐离子,再次证明了胆固醇是否翻转取决于磷脂分子是否翻转的结论。在研究hIAPP蛋白在磷脂双层膜中的结构演变动力学中,通过系统研究不同相态的磷脂膜以及抑制剂对hIAPP蛋白在生物膜中结构变化的影响,结果发现hIAPP在生物膜界面上会发生聚集,而酰胺Ⅱ谱带的出现与聚集的程度有关。
[Abstract]:In life, specific chiral molecules can perform specific functions to maintain normal life activities. It is very important to study the chirality of biomolecules at the biomembrane interface in order to understand the pathogenesis of some diseases correctly. Therefore, the further study of chiral biomolecules at the biofilm interface is helpful to understand the pathogenesis of related diseases and provide guidance for the study and synthesis of novel drugs. However, the study of chiral molecules at biofilm interface is very complicated. In this paper, two chiral biomolecules, cholesterol and hIAPP protein, were used as models of chiral, chiral and non-chiral polarization-binding sum-frequency spectroscopy. The assembly and transport behavior at the biofilm interface and the structural transformation of proteins at the biofilm interface were systematically studied. The flipping transport behavior and assembly mode of cholesterol molecules in electroneutral phospholipid membrane (PC) and negatively charged phospholipid (PG) membrane were studied. It is concluded that whether cholesterol flips in the bilayer membrane of phospholipid mainly depends on whether the phospholipid is flipped. At the same time, salt ions were introduced into PG phospholipid membrane, which proved that whether cholesterol flips depends on whether phospholipid molecules flip or not. In the study of the structural evolution kinetics of hIAPP protein in phospholipid bilayer membrane, the effects of different phase phospholipid membrane and inhibitor on the structural change of hIAPP protein in biofilm were systematically studied. The results showed that hIAPP aggregates at the interface of biofilm. The appearance of amide-II band is related to the degree of aggregation.
【学位授予单位】：中国科学技术大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;O647.1

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