QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂

发布时间：2018-05-25 01:20

本文选题：超高效液相色谱-串联质谱法 + 荧光增白剂　；参考：《分析化学》2017年05期

【摘要】：建立了QuEChERS-超高效液相色谱-串联质谱法(UPLC-MS/MS)测定食用菌中7种荧光增白剂残留量的检测方法。样品前处理采用QuEChERS方法,食用菌样品用水浸润后,以甲酸和乙腈提取目标物,C_(18)填料、正丙基乙二胺(PSA)和MgSO_4净化,C_(18)色谱柱分离,乙腈和0.1%甲酸梯度洗脱,多反应监测(MRM)正离子模式扫描。在优化条件下,7种荧光增白剂在一定质量浓度范围内线性关系良好,相关系数均大于0.991,方法检出限(S/N=3)在0.05~0.40μg/kg之间,定量低限(S/N≥10)在0.2~1.3μg/kg之间,方法的平均回收率在70.1%~109.2%之间。本方法具有操作简单快捷、灵敏度高等优点。
[Abstract]:A QuEChERS- ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was established for the determination of 7 fluorescent brighteners in edible fungi. Sample pretreatment was treated with QuEChERS. After water infiltration, the samples were extracted with formic acid and acetonitrile, C_ (18), n-propyl ethylenediamine (PSA) and MgSO_4, C_ (18) column. Separation, acetonitrile and 0.1% formic acid gradient elution, multi reaction monitoring (MRM) positive ion mode scanning. Under optimal conditions, 7 kinds of fluorescent brighteners have a good linear relationship in a certain concentration range, the correlation coefficient is more than 0.991, the detection limit (S/N=3) is between 0.05~0.40 and g/kg, and the quantitative low limit (S/N > 10) is between 0.2~1.3 and g/kg, and the method is flat. The average recovery is between 70.1%~109.2%. This method has the advantages of simple operation and high sensitivity.
【作者单位】：广东出入境检验检疫局检验检疫技术中心中山分中心;暨南大学包装工程研究所广东普通高校产品包装与物流重点实验室;
【基金】：国家质检总局科技计划项目(2015IK052,2015IK260) 广东省科技计划项目(2014A040401011) 中山市社会公益重大专项(2015B2295,2014A2FC241) 广东检验检疫局科技计划项目(2015GDK32,2017GDK24)资助
【分类号】：TS219;O657.63

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