基于UPLC-Q-Orbitrap HRMS方法的丹黄祛瘀胶囊中多种化学成分研究

发布时间：2018-05-25 15:21

本文选题：丹黄祛瘀胶囊 + 超高效液相色谱-四级杆/静电场轨道阱高分辨质谱　；参考：《中草药》2017年18期

【摘要】：目的为系统阐明复方中药制剂丹黄祛瘀胶囊中的化学成分组成,建立一种同时对中药复杂成分实现精准、快速鉴定的高效分析方法。方法本研究采用超高效液相色谱-四级杆/静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS),对丹黄祛瘀胶囊中的化学成分进行鉴定分析,通过UPLC-Q-Orbitrap HRMS扫描提供的化合物精确相对分子质量、多级碎片离子信息,同时与对照品的的相对保留时间和质谱数据进行比对,并结合相关参考文献从而实现对化合物鉴定的准确定性。结果共鉴定出59种化学成分,其中主要包括黄酮类、苯酞内酯类、醌类、单萜苷类、有机酸类及其他类。结论可系统、准确、快速地鉴定丹黄祛瘀胶囊中的多种化学成分,并为其药效物质基础及质量控制等研究提供了科学的理论依据。
[Abstract]:Objective to elucidate the chemical composition of Danhuang Quyu capsule and to establish an efficient method for accurate and rapid identification of complex components of traditional Chinese medicine at the same time. Methods the chemical constituents of Danhuang Quayu capsule were identified and analyzed by UPLC-Q-Orbitrap HRMSM with UPLC-Q-Orbitrap HRMSMS-UPLC-Q-Orbitrap HRMS.The exact relative molecular weight of the compounds was obtained by UPLC-Q-Orbitrap HRMS scanning. The multilevel fragment ion information was compared with the relative retention time of the reference substance and the mass spectrum data, and combined with the relevant references, the identification of the compounds could be accurately identified. Results A total of 59 chemical constituents were identified, including flavonoids, phthalides, quinones, monoterpenoids, organic acids and other compounds. Conclusion the chemical constituents of Danhuang Quyu capsule can be identified systematically, accurately and quickly, and it provides a scientific theoretical basis for the study of the substance basis and quality control of Danhuang Quyu capsule.
【作者单位】：郑州大学第一附属医院药学部;河南省精准临床药学重点实验室;河南理工大学医学院;郑州大学药物研究院;
【基金】：国家自然科学基金资助项目(81603287) 郑州大学第一附属医院创新基金项目(2015) 北京医卫健康公益基金项目(YWJKJJHKYII-B16240)
【分类号】：O657.63;R286.0

【相似文献】