基于毛细管电泳技术的有机磷农药新型检测方法研究

发布时间：2018-05-26 23:46

本文选题：敌百虫 + 分子印迹聚合物　；参考：《山东农业大学》2017年硕士论文

【摘要】：本研究针对蔬菜中有机磷农药的残留问题,建立了两种新型的有机磷农药检测方法:1.分子印迹磁性固相萃取与毛细管电泳联用技术检测敌百虫方法研究该研究以敌百虫为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂制备了量子点磁性分子印迹聚合物。将其作为吸附剂,建立了分子印迹磁性固相萃取和毛细管电泳技术联用(MIMSPE-CE)的快速检测农产品里敌百虫含量的新方法。最优条件下,该方法的最低检出限为0.03 mg kg-1,重复5次相对标准偏差为4.2%。空白黄瓜和菜花样品中3个浓度的添加回收率为82%~96.6%。与气相色谱测定结果具有较好的一致性。该方法还应用于油菜样品中敌百虫残留的定量分析。2.基于离子液体的乙酰甲胺磷和水胺硫磷的分离检测方法研究离子液体(ILs)和十二烷基硫酸钠(SDS)作为毛细管电泳背景电解质的修饰剂可以实现乙酰甲胺磷和水胺硫磷的分离。本实验考察了离子液体种类及浓度、SDS的浓度、缓冲液pH对乙酰甲胺磷和水胺硫磷分离效率的影响。在最优条件下,该方法对乙酰甲胺磷和水胺硫磷最低检出限为0.154 mg kg-1和0.08 mg kg-1,重复5次的峰面积的相对标准偏差为1.9%~3.9%,迁移时间的相对标准偏差为0.4%~0.6%。空白黄瓜、菜花、菠菜和胡萝卜样品的2个浓度水平的添加回收率为76.8%~88.8%。
[Abstract]:In order to solve the problem of organophosphorus pesticide residues in vegetables, two new methods for the determination of organophosphorus pesticides: 1 were established. Detection of trichlorfon by Molecular imprinted Magnetic solid Phase extraction and Capillary Electrophoresis the study used trichlorfon as template molecule and methacrylic acid as functional monomer. Quantum dot magnetic molecularly imprinted polymer was prepared by using ethylene glycol dimethacrylate as crosslinking agent. A new method for rapid determination of trichlorfon in agricultural products by molecular imprinted magnetic solid phase extraction (MIMSPE-CEE) and capillary electrophoresis was established. Under the optimum conditions, the detection limit of the method is 0.03 mg 路kg ~ (-1), and the relative standard deviation of the method is 4.2 times. The recoveries of the three concentrations in the blank cucumber and cauliflower samples were 82. 6% and 96. 6% respectively. The results are in good agreement with the results of gas chromatography. The method was also applied to quantitative analysis of trichlorfon residues in rape samples. Separation and Detection of Ionic liquid based on Acetamidophos and Isophosphorus; Ionic liquids and Sodium dodecyl Sulfate) can be used as modifiers for background electrolyte in capillary electrophoresis to separate acetamidophos from water-sulphos. The effects of the concentration of ionic liquids, concentration of SDS and pH of buffer solution on the separation efficiency of acephate and water-sulphos were investigated. Under the optimum conditions, the minimum detection limits for methamidophos and methamidophos were 0.154 mg kg-1 and 0.08 mg kg-1, the relative standard deviation for the peak area repeated five times was 1.9%, and the relative standard deviation for migration time was 0.4 mg / kg ~ (-1). The recoveries of two concentration levels of cucumber, cauliflower, spinach and carrot were 76.8and 88.8.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O658.9;TS255.7

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