罗丹明类重金属离子荧光探针的合成与应用

发布时间：2018-05-31 03:50

本文选题：罗丹明 + 荧光探针　；参考：《海南师范大学》2017年硕士论文

【摘要】：当今,环境中的重金属污染问题已日益加重,一些重金属在人和动物体内扮演了很重要的角色。在众多重金属离子检测方法中,荧光探针法具有灵敏度高、特异性强、方便快捷等特点。罗丹明类荧光染料具有毒性小、水溶性好、光量子产率高等优点。因此研究者广泛的关注了罗丹明类荧光分子探针。本文概述了罗丹明类荧光分子探针的研究进展,在此基础上,以罗丹明衍生物作为母体,主要设计合成了3种荧光探针。具体内容如下:(1)一种基于丹酰氯与罗丹明B的新型荧光探针。可以通过显示独特的荧光和吸收特性从其他常见金属离子中选择性识别Hg~(2+)和Cu~(2+)。在MeCN/HEPES缓冲溶液中,探针对Hg~(2+)具有比例荧光响应,这归因于从丹酰基到开环的罗丹明B的荧光共振能量转移,但当Cu~(2+)的存时候能引起荧光猝灭,其他金属离子对该探针没有影响。(2)通过亚胺键连接罗丹明6G酰肼与N-甲基靛红合成了一个新的荧光探针,可以选择性识别和传感Pb~(2+)、Hg~(2+)和Cd~(2+)。在乙醇/HEPES缓冲溶液中,加入Cd~(2+)导致黄绿荧光,而Pb~(2+)或Hg~(2+)的存在产生橙色荧光。此外,探针显示对Pb~(2+)的不可逆荧光响应和对Hg~(2+)和Cd~(2+)的可逆荧光响应。(3)用乙二胺连接罗丹明B和7-二乙胺基-3-醛基香豆素合成了一个荧光共振能量转移系统RC,合成方法简便、快速,产物经核磁共振、质谱和X-射线单晶衍射确认。
[Abstract]:Nowadays, the pollution of heavy metals in the environment is becoming more and more serious, and some heavy metals play an important role in human and animal. Among many heavy metal ion detection methods, the fluorescence probe method has the characteristics of high sensitivity, specificity, convenience and so on. Rhodamine fluorescent dyes have the advantages of low toxicity, good water solubility and high light quantum yield. So researchers have focused extensively on Rhodamine fluorescent probes. In this paper, the research progress of Rhodamine fluorescent molecular probes is summarized. On the basis of this, three kinds of fluorescent probes are designed and synthesized with Rhodamine derivatives as the parent. A novel fluorescent probe based on Danyl chloride and rhodamine B. Hg~(2 and Cu~(2 can be selectively identified from other common metal ions by displaying unique fluorescence and absorption characteristics. In MeCN/HEPES buffer solution, the probe has a proportional fluorescence response to Hg~(2, which is attributed to the fluorescence resonance energy transfer from the Danyl group to the open ring Rhodamine B, but it can cause fluorescence quenching when the Cu~(2) exists. Other metal ions had no effect on the probe. 2) A new fluorescent probe was synthesized by linking Rhodamine 6G hydrazide with N-methylindirubin by imine bond, which can selectively recognize and sensing the Pb~(2 and Cd~(2. In ethanol / HEPES buffer solution, the addition of Cd~(2) leads to yellowish green fluorescence, while the presence of Pb~(2) or Hg~(2) produces orange fluorescence. In addition, The probe showed irreversible fluorescence response to Pb~(2 and reversible fluorescence response to Hg~(2 and Cd~(2).) A fluorescence resonance energy transfer system was synthesized by using ethylenediamine to link rhodamine B and 7-diethylamino -3-aldehyde coumarin. The method is simple and convenient. The product was confirmed by NMR, MS and X-ray single crystal diffraction.
【学位授予单位】：海南师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3

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