高效阴离子交换色谱-脉冲安培检测法测定多花黄精多糖的单糖组成

发布时间：2018-05-31 13:37

本文选题：多花黄精 + 多糖　；参考：《中草药》2017年08期

【摘要】：目的建立高效阴离子交换色谱-脉冲安培检测法(HPAEC-PAD)测定多花黄精多糖的单糖组成。方法多花黄精多糖经提取后,用硫酸水解,用Carbo Pac PA1色谱柱(250 mm×4 mm,10.0μm)进行分离,采用梯度洗脱,HPAEC-PAD测定多花黄精多糖中单糖的组成。结果测得各单糖在1~100 mg/L线性关系良好(r0.999),最低检出限为0.015~0.025 mg/L,RSD为1.35%~2.80%,加样回收率为88.36%~111.3%。结果表明不同产地的多花黄精多糖均由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖和果糖组成。结论本实验所建立的方法分离效果好,灵敏度高,可用于多花黄精多糖的单糖组成和测定的分析研究。
[Abstract]:Objective to establish a high performance anion exchange chromatography-pulse amperometric method (HPAEC-PAD) for the determination of monosaccharide composition of Polygonatum polygonatum polysaccharide. Methods Polysaccharide of Polygonatum polygonatum was extracted and hydrolyzed with sulfuric acid and separated by Carbo Pac PA1 column (250mm 脳 4mm). The composition of monosaccharide in polysaccharide was determined by gradient elution HPAEC-pad. Results the linear relationship of each monosaccharide was good at 1 ~ 100 mg/L, the lowest detection limit was 0.015 ~ 0.025 mg / L ~ (-1) and the average recovery was 88.36% ~ 111.3%. The results showed that the polysaccharides from different producing areas were composed of rhamnose, arabinose, galactose, glucose, mannose and fructose. Conclusion the method is effective and sensitive, and can be used for the analysis of monosaccharide composition and determination of Polygonatum polygonatum polysaccharides.
【作者单位】：杭州职业技术学院;磐安县人民医院;杭州娃哈哈集团有限公司研究院;
【基金】：浙江省科技厅分析测试科技计划项目(2015C37020)
【分类号】：R284;O657.7

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