维生素C荧光光谱的机理研究

发布时间：2018-06-04 21:38

本文选题：维生素C + 荧光光谱　；参考：《食品工业科技》2016年22期

【摘要】：以V_C标品为研究对象,利用荧光分光光度计分别采集了280~350 nm激发波长下的荧光发射光谱,结果表明:V_C溶液的最大荧光峰位于EX310 nm/EM400 nm,VC光谱的二阶导数有4个极值点和3个拐点,分别为:328、345、355、372、395、425、525 nm。通过理论分析,证实395 nm是-OH发生的n→σ*电子跃迁,二烯醇结构π→π*电子跃迁产生的荧光峰由于共轭体系的作用发生红移而位于425、525 nm。分子中的各个荧光团相互叠加且彼此制约,在分子团簇作用和水分子的H键的共同作用下,最终使得V_C溶液在400 nm有最大荧光峰。
[Abstract]:Taking the VMC standard as the research object, the fluorescence emission spectra at 280 ~ 350nm excitation wavelength were collected by fluorescence spectrophotometer. The results show that the maximum fluorescence peak of the solution is located at four extreme points and three inflection points of the second derivative of the EX310 nm/EM400 NMMC VC spectrum. They are respectively: 328345355372395425525 nm. Through theoretical analysis, it is proved that 395nm is the n-蟽 * electron transition of -OH, and the fluorescence peak produced by 蟺 ~ * electron transition of dienol structure is located at 425525 nm because of the red shift of conjugated system. Each fluorescence group in the molecule superposes and restricts each other. Under the interaction of the molecular cluster and the H bond of the water molecule, the maximum fluorescence peak of V _ S _ C solution at 400 nm is finally obtained.
【作者单位】：宁夏大学农学院;
【基金】：国家自然科学基金项目(31560481)
【分类号】：O629.4;O657.3

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