荧光碳量子点的微波法制备光学性能及其应用研究

发布时间：2018-06-08 22:44

本文选题：碳量子点 + 微波法　；参考：《延安大学》2017年硕士论文

【摘要】：碳量子点(Carbon Quantum Dots—CQDs)这一新型纳米材料越来越被科研工作者重视,它不仅具有传统纳米材料所具备的所有性质,而且还具有许多优越特性,比如它的低细胞毒性和良好的生物相容性使得它在生命科学领域已不可或缺。经过科研工作者们多年的努力,CQDs在荧光生物标记方面的研究得以大踏步前进,除此之外,CQDs在离子检测、药物检测中也有着广泛的应用,以CQDs为荧光探针来定量测定离子和药物的新方法越来越多,这些方法更为简单、快捷、灵敏度高,而且大多数已经运用到实际样品检测。本论文选取廉价易得的碳源,用简捷的微波法合成荧光性能较好的CQDs,并试图对合成CQDs的结构、荧光性质进行分析,探讨了其合成作用机理,同时以CQDs为荧光探针检测了金属离子和药物小分子,探究了CQDs与离子和药物小分子之间作用机理,主要内容可以分为四部分。1.通过微波法,以D-麦芽糖、D-果糖为碳源合成了CQDs。对合成的CQDs进行了结构表征、光学特性分析及潜在应用探讨。结果证明,此类CQDs表面富有羟基和羧基,水溶性好,分布均匀。在弱酸性环境下具有良好的荧光性能,一些氧化还原剂、金属离子对其荧光强度有一定的增敏和猝灭作用,但表面活性剂对其荧光性能影响不大,这为其应用奠定了良好的基础。2.以D-麦芽糖为碳源合成的CQDs为荧光探针检测延河水中痕量的Co2+,并进行了相互作用机理探讨。实验结果表明,该CQDs对Co2+具有较好的选择性,体系的荧光猝灭强度与Co2+的浓度在0.03?g/mL~0.6?g/mL及0.6?g/mL~4?g/mL范围内呈良好的线性关系,检出限为0.00384?g/mL,回收率在98.8%-103.3%之间,说明该方法的灵敏度及准确度较高。3.在pH=4.80的柠檬酸-柠檬酸钠缓冲介质中,建立了以CQDs为荧光探针定量测定曲克芦丁的新方法。结果表明,当曲克芦丁的浓度在1.0×10-7~3.0×10-5mol/L的范围内时,与CQDs的荧光猝灭强度呈良好的线性,检出限为4.5×10-9mol/L,且发现二者之间的作用机理为动态猝灭。该方法已成功用于曲克芦丁药片的测定,结果满意。4.在pH=5.00的B-R的缓冲溶液中,以CQDs为荧光探针定量测定盐酸雷尼替丁,建立了一种快速、选择性好的检测盐酸雷尼替丁的新方法。实验发现,盐酸雷尼替丁浓度在1.0×10-6~7.0×10-5 mol/L和1.0×10-7~1.0×10-6 mol/L范围内与体系荧光猝灭程度呈现良好的线性关系,检出限为7.1×10-8 mol/L。对实际药品进行含量测定,回收率为96.4%~99.0%,并探讨了其猝灭机理为动态猝灭。
[Abstract]:Carbon Quantum Dot (CQDs), a new nanometer material, has been paid more and more attention by researchers. It not only has all the properties of traditional nanomaterials, but also has many superior properties. For example, its low cytotoxicity and good biocompatibility make it indispensable in life sciences. After many years of efforts by researchers, the study of CQDs in fluorescent biomarkers has made great strides forward. In addition, CQDs have been widely used in ion detection and drug detection. There are more and more new methods for quantitative determination of ions and drugs using CQDs as fluorescent probes. These methods are simpler, faster and more sensitive, and most of them have been applied to the detection of real samples. In this paper, CQDs with good fluorescence properties were synthesized by simple microwave method, and the structure and fluorescence properties of synthesized CQDs were analyzed, and the mechanism of the synthesis was discussed. At the same time, metal ions and drug molecules were detected with CQDs as fluorescence probe, and the mechanism of interaction between CQDs and ions and drug small molecules was explored. The main contents can be divided into four parts. 1. CQDs were synthesized by microwave method using D-maltose and D-fructose as carbon source. The structures, optical properties and potential applications of synthesized CQDs were investigated. The results show that the surface of CQDs is rich in hydroxyl and carboxyl groups, and has good water solubility and uniform distribution. Some oxidizing reductants and metal ions have some sensitizing and quenching effects on the fluorescence intensity, but surfactants have little effect on the fluorescence properties. This has laid a good foundation for its application. CQDs synthesized with D-maltose as carbon source were used as fluorescence probe to detect trace Co _ 2 in Yanhe River water and the interaction mechanism was discussed. The experimental results show that the CQDs have a good selectivity for CO2. The fluorescence quenching intensity of the system is linear with the concentration of CO2 in the range of 0.03g / mL and 0.6g / mL, and the detection limit is 0.00384g / mL and the recovery is between 98.8- 103.3%, and the detection limit is 0.00384g / mL, and the recovery rate is between 98.8- 103.3%, and the detection limit is 0.00384g / mL, and the recovery is between 98.8- 103.3%, and the detection limit is 0.00384g / mL, and the recovery is between 98.8- 103.3%. It shows that the sensitivity and accuracy of this method are high. 3. A new method for quantitative determination of troxerutin with CQDs as fluorescence probe was established in citric acid-sodium citrate buffer medium with pH = 4.80. The results show that when the concentration of troxerutin is in the range of 1.0 脳 10-7, 3.0 脳 10-5 mol / L, the fluorescence quenching intensity with CQDs is linear, the detection limit is 4.5 脳 10-9 mol / L, and the mechanism of the interaction is dynamic quenching. The method has been successfully applied to the determination of troxerutin tablets with satisfactory results. A new rapid and selective method for quantitative determination of ranitidine hydrochloride with CQDs as fluorescence probe was established in the buffer solution of B-R at pH 5.00. It was found that the concentration of ranitidine hydrochloride showed a good linear relationship with the fluorescence quenching in the range of 1.0 脳 10 ~ (-6) ~ 7.0 脳 10 ~ (-5) mol / L and 1.0 脳 10 ~ (-7) mol / L, and the detection limit was 7.1 脳 10 ~ (-8) mol 路L ~ (-1). The content of real medicine was determined. The recovery rate was 96.4% and 99.0. The quenching mechanism was discussed as dynamic quenching.
【学位授予单位】：延安大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O657.3

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