导电聚合物纳米线复合材料的制备及其电化学生物传感研究

发布时间：2018-06-10 15:46

本文选题：导电聚合物 + 聚苯胺纳米线　；参考：《青岛科技大学》2017年硕士论文

【摘要】：本课题采用电化学方法,在电极上成功制备了不同的新型导电聚合物纳米线复合材料。然后,基于这些纳米线复合材料构建了几种检测不同肿瘤标志物的电化学免疫传感器。通过扫描电子显微镜、光电子能谱、电化学阻抗和微分脉冲伏安法等方法对这些纳米线复合材料的微观结构、元素组成、电化学性能和抗污染能力进行了表征。我们希望这些新型的生物传感平台能够为实际临床检测提供可靠的技术支持。本课题的主要工作简述如下:(1)制备了一种新型的基于聚乙二醇/金纳米粒子/聚苯胺纳米线(PEG/AuNPs/PANI)的电化学免疫传感器。该传感器以聚苯胺纳米线(PANI)为基底,继而修饰上金纳米粒子(AuNPs)和聚乙二醇(PEG)。制备的新型PEG/AuNPs/PANI复合材料,结合了AuNPs/PANI的优良的电导率和PEG良好的抗污染能力,为具有抗污染能力的电化学免疫传感器的发展提供的一个理想的基底。甲胎蛋白(AFP)是众所周知的肝癌的肿瘤标志物,将其作为检测对象,把它的抗体固定到PEG/AuNPs/PANI复合材料上,构建成一个免标记、抗污染的检测AFP的免疫传感器。通过使用聚苯胺的氧化还原电流作为传感信号,结合PEG/AuNPs良好的生物相容性和PEG优异的抗污染能力,使得该传感器有较宽的线性范围,最低检测限达到0.007 pg mL~(-1)。(2)制备了一种无试剂、免标记的用来检测结肠直肠癌的重要肿瘤标志物癌胚抗原(CEA)的电化学免疫传感器。在聚3,4-乙烯二氧噻吩(PEDOT)掺杂离子液体(IL)1-乙基-3-甲基咪唑双三氟甲磺酰亚胺盐的多孔复合材料上,电化学沉积聚苯胺纳米线制备得到网状复合材料。该复合材料的特点是有良好的导电性和比表面积大的多孔结构。在该复合材料修饰的玻碳电极上,固定癌胚抗原的抗体以检测癌胚抗原。该免疫传感器的线性范围为0.001-10 ng m L~(-1),最低检测限达到0.7 pg mL~(-1),同时它还具有良好的稳定性和选择性。(3)在这项工作中,发展了一种新型的通过剪切特定序列的多肽(peptide)来分析前列腺癌抗原的电化学方法。首先,在磷酸盐缓冲溶液中通过电化学方法合成聚吡咯纳米线(Ppy)。然后,在聚吡咯纳米线上自组装金纳米粒子(AuNPs)用来固定具有抗污染性能的多肽。制备好的peptide/AuNPs/Ppy新型复合材料修饰电极,结合了AuNPs/Ppy优良的导电性和peptide良好的抗污染能力,为拥有优异抗污染能力的电化学传感器的发展提供的一个极好的基质。在前列腺癌抗原的存在下,多肽序列被其特异性的识别并剪切,由此导致的电极阻抗变化可以用于前列腺癌抗原的检测。在最佳的实验条件下,该免疫传感器的线性范围为0.00001-10 ng mL~(-1)。
[Abstract]:In this paper, different conductive polymer nanowire composites were successfully prepared on the electrode by electrochemical method. Then, based on these nanowire composites, several electrochemical immunosensors were constructed to detect different tumor markers. The microstructure, elemental composition, electrochemical performance and anti-pollution ability of these nanowires were characterized by scanning electron microscopy, photoelectron spectroscopy, electrochemical impedance and differential pulse voltammetry. We hope these new biosensor platforms can provide reliable technical support for practical clinical detection. A novel electrochemical immunosensor based on PEG / au nanoparticles / Polyaniline nanowires PEG / AuNPs / PANIs was prepared. The sensor is based on Polyaniline nanowires (PANI) and then modified with au nanoparticles (AuNPs) and polyethylene glycol (PEGN). The new PEG / AuNPs / pani composite, which combines the excellent conductivity of AuNPs / pani and the good anti-pollution ability of PEG, provides an ideal substrate for the development of electrochemical immunosensors with anti-pollution ability. AFP (alpha-fetoprotein) is a well-known tumor marker of liver cancer. Its antibody was immobilized on PEG / AuNPs / pani composite to construct an anti-AFP immunosensor. By using the redox current of Polyaniline as the sensing signal, combining the good biocompatibility of PEG / AuNPs and the excellent anti-pollution ability of PEG, the sensor has a wide linear range and the minimum detection limit is 0.007 PG / mL ~ (-1). Electrochemical immunosensors for detection of carcinoembryonic antigen (CEA), an important tumor marker in colorectal cancer. The composite was prepared by electrochemical deposition of Polyaniline nanowires on porous composites doped with poly (3-trimethylidene dioxothiophene) (PEDOT) ionic liquid, 1-ethyl-3-methyl imidazolium bis (fluoromethylsulfonimide), and prepared by electrochemical deposition of Polyaniline nanowires. The composite is characterized by good electrical conductivity and large specific surface area porous structure. Antibodies against carcinoembryonic antigen were immobilized on the composite modified glassy carbon electrode to detect carcinoembryonic antigen. The linear range of the immunosensor is 0.001-10 ng / mL ~ (-1), the minimum detection limit is 0.7 PG / mL ~ (-1), and it also has good stability and selectivity. A novel electrochemical method for the analysis of prostate cancer antigens by shearing a specific sequence of peptide peptides has been developed. Firstly, polypyrrole nanowires were synthesized by electrochemical method in phosphate buffer solution. Self-assembled au nanoparticles (AuNPs) were then used to immobilize polypeptides with antifouling properties on polypyrrole nanowires. The new composite modified electrode prepared by Peptide / AuNPs / Ppy combines the excellent conductivity of AuNPs / Ppy and the excellent anti-pollution ability of peptide, which provides an excellent substrate for the development of electrochemical sensors with excellent anti-pollution ability. In the presence of prostate cancer antigen, polypeptide sequences are specifically identified and cut, resulting in changes in electrode impedance can be used to detect prostate cancer antigen. Under the optimum experimental conditions, the linear range of the immunosensor was 0.00001-10 ng / mL ~ (-1).
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.1

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