基于计时库仑技术的可再生型三磷酸腺苷适配体电化学传感器的研究

发布时间：2018-06-12 09:21

本文选题：核酸适配体 + 计时库仑　；参考：《分析化学》2017年05期

【摘要】：构建了一种可再生型三磷酸腺苷(ATP)适配体计时库仑电化学传感器。将一条短链DNA通过Au-S键自组装固定在电极表面,ATP的核酸适配体与该短链DNA杂交而结合在电极表面。带负电的DNA通过静电吸引结合电解液中的六氨合钌(RuHex)阳离子。当传感器和靶分子ATP孵育后,ATP与核酸适配体结合,使适配体链从电极表面解离,电极表面吸附的DNA量减少,结合Ru Hex的量随之降低。通过计时库仑技术检测Ru Hex响应信号降低的量,可以对ATP进行定量测定。此传感器的电化学响应信号与ATP浓度对数值呈线性关系,线性检测范围为0.001~100μmol/L,检出限(S/N=3)为0.5 nmol/L。此传感器检测靶分子ATP后,可以通过简单的操作步骤再生,再生5次后的响应信号为初始信号的90%以上。采用此传感器检测大鼠脑透析液中ATP的含量为(19.2±3.7)nmol/L(n=3)。
[Abstract]:A regenerative timing Coulomb electrochemical sensor for adenosine triphosphate (ATP) aptamer was constructed. A nucleic acid aptamer fixed on the electrode surface by Au-S bond was hybridized with the short strand DNA to bind to the electrode surface. Negatively charged DNA attracts RuHexcations from the binding electrolyte by electrostatic attraction. When the sensor and target molecule ATP are incubated, the ATP binds to the aptamer of nucleic acid, which results in the dissociation of the aptamer chain from the electrode surface, the decrease of the amount of DNA adsorbed on the electrode surface and the decrease of the binding amount of Ru Hex. The reduction of Ru Hex response signal can be detected by timing Coulomb technique, and ATP can be measured quantitatively. The electrochemical response signal of the sensor has a linear relationship with the logarithmic value of ATP concentration. The linear detection range is 0.001 渭 mol / L, and the detection limit is 0.5 nmol / L. After detecting target molecule ATP, the sensor can be regenerated by simple operation step, and the response signal after 5 times regeneration is more than 90% of the initial signal. The content of ATP in rat brain dialysate was 19.2 卤3.7nmol / L ~ (-1) nmol / L ~ (3 +).
【作者单位】：安徽农业大学茶树生物学与资源利用国家重点实验室;安徽农业大学理学院应用化学系安徽农业大学;
【基金】：国家自然科学基金项目(No.21305002) 安徽农业大学人才项目(No.yj2015-28)资助~~
【分类号】：O657.1

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