槲皮素与芦丁电化学分离检测的研究

发布时间：2018-06-15 16:55

本文选题：槲皮素 + 芦丁　；参考：《合肥工业大学》2017年硕士论文

【摘要】：槲皮素和芦丁是自然界中存在的最丰富的天然黄酮类抗氧化剂,其中芦丁是槲皮素的芸香糖苷,两者通常共存于许多植物中。文献中已有对两者进行同时电化学检测的报道,但由于两者氧化峰的重叠而遇到困难。本文内容包括两部分,分别通过改进常规电化学方法和采用全新的双极电化学方法对槲皮素和芦丁进行电化学分离检测。将石墨片电极进行预氧化活化处理,对槲皮素和芦丁的电化学反应过程进行伏安研究,了解其反应机理,并为优化电化学分离检测提供参考。另一方面,通过改变支持电解质浓度,以增强槲皮素和芦丁在电极上的吸附及电氧化信号。对天然抗氧化剂的电化学研究,本身也有助于理解其抗氧化活性及相关的药理作用机制。以电化学预处理的石墨片为工作电极,对槲皮素的多步氧化反应进行了伏安扫描测试,得到了 4个阳极峰和6个反向阴极峰,电流峰的数目多于文献报道;考察了电势扫描范围、电解液pH值、富集时间、扫描速率、槲皮素浓度等对各伏安电流峰的影响;提出了槲皮素第1和第3阳极峰的连串氧化反应机理,使对槲皮素这一重要的天然黄酮化合物的氧化代谢机理的认识深化了一步。结果表明经氧化预处理的石墨片电极的性能优于未经过氧化预处理的石墨片电极。再用石墨片电极对芦丁的多步氧化反应进行实验,实验结果得到了芦丁的两个增强的氧化峰。增大支持电解质浓度,也达到了增强槲皮素和芦丁的电氧化信号的目的。但两者的电氧化峰的重叠问题未得到改善。基于上述,尝试运用双极电化学原理设计全新的薄层微流控双极电化学电解池,旨在利用微通道中溶液中存在的梯度电势差,对槲皮素和芦丁进行分离检测。根据测试结果,对双极电化学微通道流动反应池的结构进行了持续改进,逐步解决了双极池运行过程中出现的一些困难和技术问题。将该方法应用于多组分电化学分离检测的思路具有很强的探索性,目前尚未能达到最终的目标,但为后续的改进提供了经验和信心。
[Abstract]:Quercetin and rutin are the most abundant natural flavonoids antioxidants in nature, among which rutin is the rutin glycoside of quercetin, which usually coexist in many plants. The simultaneous electrochemical detection of both has been reported in the literature, but it is difficult because of the overlap of oxidation peaks. This paper consists of two parts. The electrochemical separation of quercetin and rutin is carried out by improving the conventional electrochemical method and using a new bipolar electrochemical method. The electrochemical reaction of quercetin and rutin was studied by voltammetry. The mechanism of the reaction was understood and the reference was provided for the optimization of electrochemical separation and detection. On the other hand, the adsorption and electrooxidation signals of quercetin and rutin on the electrode were enhanced by changing the concentration of supporting electrolyte. The electrochemical study of natural antioxidants also helps to understand their antioxidant activity and related pharmacological mechanisms. The multistep oxidation reaction of quercetin was measured by voltammetry with graphite sheet pretreated by electrochemical method. Four anodic peaks and six reverse cathodic peaks were obtained, and the number of current peaks was more than that reported in literature. The effects of potential scanning range, electrolyte pH value, enrichment time, scanning rate and quercetin concentration on the volt-ampere current peaks were investigated, and the mechanism of successive oxidation of the first and third anodic peaks of quercetin was proposed. The mechanism of oxidation metabolism of quercetin, an important natural flavonoid, is further understood. The results show that the performance of the graphite sheet electrode pretreated by oxidation is better than that of the graphite sheet electrode without preoxidation. The multistep oxidation reaction of rutin was carried out with graphite electrode. Two enhanced oxidation peaks of rutin were obtained. Increasing the concentration of supporting electrolyte also enhances the electrooxidation signal of quercetin and rutin. However, the overlap of the two electrooxidation peaks has not been improved. Based on the above, a novel thin-layer microfluidic bipolar electrolysis cell was designed by using the bipolar electrochemistry principle. The aim was to separate quercetin from rutin by using the gradient potential difference in the solution of the microchannel. According to the test results, the structure of the bipolar electrochemical microchannel flow reaction cell has been continuously improved, and some difficulties and technical problems in the operation of the bipolar cell have been solved step by step. The idea of applying this method to multicomponent electrochemical separation and detection is very exploratory, but it can not reach the final goal yet, but it provides experience and confidence for further improvement.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.1;TQ28

【参考文献】