新型鲁米诺化学发光体系的研究以及其在过氧化氢和葡萄糖检测中的应用

发布时间：2018-06-24 04:50

本文选题：过氧化氢 + 银纳米簇　；参考：《天津大学》2016年硕士论文

【摘要】：化学发光分析法是根据当CL进行到某一时刻时,依据发光量子总数或发光强度来对被分析组分进行定量分析的一种微量或痕量的分析方法。化学发光分析法具有操作简单,灵敏度高和仪器设备简单等优点。鲁米诺-过氧化氢化学发光体系,一种经典的化学发光反应,已经被广泛的应用于过氧化氢和葡萄糖的检测。但是鲁米诺-过氧化氢体系产生CL强度非常的微弱,很多催化剂包括过渡金属离子、纳米粒子和生物酶等被用来作为鲁米诺-过氧化氢化学发光体系的催化剂。在这些催化剂中,辣根过氧化物酶(HRP)能够显著的增强鲁米诺-过氧化氢化学发光体系的化学发光,然而,作为生物酶,HRP具有易失活,价格昂贵和制备纯化过程费时等缺点。因此,寻找更为优异的催化剂或增敏剂的研究具有重要意义。在我们的研究中发现,四碘酚磺酞和银纳米簇能够显著地催化鲁米诺-过氧化氢化学发光,使其CL信号增强。全文分为两个部分:第一章基于四碘酚磺酞催化鲁米诺化学发光和应用于过氧化氢和葡萄糖的检测。本章基于四碘酚磺酞催化鲁米诺-过氧化氢化学发光体系,建立了一种全新的定量分析过氧化氢和葡萄糖的化学发光分析法。第一步,建立了四碘酚磺酞催化的鲁米诺-过氧化氢化学发光体系,并初步探索了四碘酚磺酞对鲁米诺-过氧化氢化学发光体系的催化机理;第二步,以过氧化氢为被分析物,将鲁米诺-过氧化氢-四碘酚磺酞化学发光体系用于过氧化氢和葡萄糖的定量分析。在实验条件被优化的条件下,检测过氧化氢的线性范围为0.025-10μM,检测限为14 nM,检测葡萄糖的线性范围为0.1-30μM。最后,将四碘酚磺酞催化的鲁米诺-过氧化氢化学发光体系应用于人血清中葡萄糖的检测。第二章基于银纳米簇催化鲁米诺化学发光和应用于过氧化氢和葡萄糖的定量分析。本章基于银纳米簇催化鲁米诺-过氧化氢化学发光体系,构建了高灵敏度检测过氧化氢和葡萄糖的分析方法。在最佳实验条件下,检测过氧化氢的线性范围为0.025-75μM,检测限为1.7 nM。检测葡萄糖的线性范围为0.01-50μM,检测限为7.2 nM。
[Abstract]:Chemiluminescence analysis is a micro or trace analytical method for quantitative analysis of analyzed components according to the total number or intensity of luminescent quantum when CL is carried out at a certain time. Chemiluminescence analysis has the advantages of simple operation, high sensitivity and simple equipment. Luminol-hydrogen peroxide chemiluminescence system, a classical chemiluminescence reaction, has been widely used in the detection of hydrogen peroxide and glucose. However, the CL intensity of Lumino-hydrogen peroxide system is very weak. Many catalysts, such as transition metal ions, nanoparticles and biological enzymes, are used as catalysts for luminol hydrogen peroxide chemiluminescence system. Among these catalysts, horseradish peroxidase (HRP) can significantly enhance the chemiluminescence of luminol hydrogen peroxide chemiluminescence system. However, HRP as a biological enzyme has the disadvantages of easy inactivation, high cost and time-consuming preparation and purification. Therefore, it is of great significance to search for better catalysts or sensitizers. We found that tetraiodophenolsulfonphthalein and silver nanoclusters can catalyze luminol hydrogen peroxide chemiluminescence and enhance CL signal. The first chapter is based on the chemiluminescence of luminol catalyzed by tetraiodophenolsulfonphthalein and its application in the detection of hydrogen peroxide and glucose. In this chapter, a novel chemiluminescence method for quantitative analysis of hydrogen peroxide and glucose was developed based on the chemiluminescence system of luminol and hydrogen peroxide catalyzed by tetraiodophenol sulfophthalein. In the first step, a luminol hydrogen peroxide chemiluminescence system catalyzed by tetraiodophenol sulfophthalein was established, and the catalytic mechanism of luminol sulfophthalein for the luminol hydrogen peroxide chemiluminescence system was preliminarily explored. The luminol-hydrogen peroxide-tetraiodophenol sulfonphthalein chemiluminescence system was applied to the quantitative analysis of hydrogen peroxide and glucose. Under the optimized conditions, the linear range of detection of hydrogen peroxide is 0.025-10 渭 M, the detection limit is 14 nm, and the linear range of glucose detection is 0.1-30 渭 M. Finally, the luminol-hydrogen peroxide chemiluminescence system catalyzed by tetraiodophenol sulfophthalein was applied to the determination of glucose in human serum. The second chapter is based on silver nanoclusters catalyzing luminol chemiluminescence and quantitative analysis of hydrogen peroxide and glucose. In this chapter, a high sensitivity method for the determination of hydrogen peroxide and glucose was constructed based on the chemiluminescence system of Luminol and hydrogen peroxide catalyzed by silver nanoclusters. Under the optimum experimental conditions, the linear range of detection of hydrogen peroxide is 0.025-75 渭 M and the detection limit is 1.7 nm. The linear range of glucose detection was 0.01-50 渭 M and the detection limit was 7.2 nm.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3;R446.1

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