可视化分子探针的设计、合成及其对食品中氰化物的检测

发布时间：2018-06-28 13:21

本文选题：氰化物 + 食品　；参考：《食品科学》2017年12期

【摘要】：以萘酰亚胺偶氮苯为检测信号基团,以水杨醛为氰根离子的识别基团,通过将萘酰亚胺重氮盐和水杨醛单元偶联,得到基于萘酰亚胺的偶氮苯染料分子探针。研究发现,探针溶液与氰化物反应后,紫外光谱吸收峰从波长404 nm转移至505 nm,颜色从黄色变为红色。在水溶液体系中,探针分子能实现对氰化物的比值式检测,线性范围为0.5~40.0μmol/L,检出限为0.13μmol/L,远低于世界卫生组织规定饮用水中的氰化物最大含量(1.9μmol/L)。且该探针分子具有很强的特异性,对CN~-的检测不受F~-、Cl~-、Br~-、I~-、SCN~-、Ac O~-、S~(2-)、H_2PO_4~-、N_3~-、NO_3~-等共存阴离子的影响。在对实际食物样品中氰化物进行检测时,该方法的准确度与国标方法一致。另外,利用探针分子制备了氰化物的检测试纸,可直接目测出含氰化物浓度大于5.0μmol/L的样品溶液。
[Abstract]:Using naphthalimide azobenzene as the detection signal group and salicylaldehyde as the cyanide recognition group, the naphthalenimide diazo salt and salicylic aldehyde unit were coupled to obtain a naphthalene imide based azobenzene dye molecular probe. It was found that after the probe solution reacted with cyanide, the absorption peak of UV spectrum was shifted from 404 nm to 505 nm, and the color changed from yellow to red. In aqueous solution system, the probe molecule can be used to detect cyanide by the ratio formula. The linear range is 0.5 渭 mol / L and the detection limit is 0.13 渭 mol / L, which is much lower than the maximum content of cyanide in drinking water specified by WHO (1.9 渭 mol / L). Moreover, the probe molecule has a strong specificity, and the detection of CNN- is not affected by the co-existing anions such as FTX, Cl-C, Br-CU, I-, SCN-, SCN-, Ac, OO-, S2-, H2PO4-, H2PO4-, N2PO4-, N3-, and so on, which are not affected by the co-existing anions such as the co-existing anions. The accuracy of this method is consistent with that of the national standard method in the determination of cyanide in real food samples. In addition, the cyanide detection paper was prepared by using probe molecule, and the sample solution containing cyanide concentration greater than 5.0 渭 mol / L could be directly detected.
【作者单位】：湖南省食品质量监督检验研究院;商丘师范学院化学化工学院;
【基金】：湖南省食品药品监督管理局食品药品安全科技项目(湘食药科R201516) 国家自然科学基金联合基金项目(U1404215)
【分类号】：O657.32;TS207.3

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