用于快速检测沙丁胺醇的分子印迹传感器的制备及其在食品检测中的应用

发布时间：2018-06-29 13:22

  本文选题：沙丁胺醇 + 纳米金　； 参考：《现代食品科技》2017年02期

【摘要】：首先通过电沉积纳米金,电聚合聚硫堇对玻碳电极表面进行了修饰,之后利用沙丁胺醇为模板分子,通过电聚合α-甲基丙烯酸,制备了一种选择性具有特异识别功能的纳米金/聚硫堇/沙丁胺醇分子印迹电化学传感器。实验采用循环伏安法和差分脉冲法,对不同的修饰电极进行了表征,优化电极的制备和测定条件,研究了印迹传感器对模板分子的选择性响应。结果表明:最佳制备条件下,模板分子:功能单体=1:4,聚合15圈,以0.5 mmol/L硫酸:乙腈(1:9,V/V)洗脱10 min,在p H=7.0的PBS溶液中进行DPV检测,线性范围为2.0×10-7~1.0×10-4 mol/L,检测限为6.0×10-8 mol/L,S/N=3。该传感器灵敏度高,抗干扰能力强,重现和稳定性好。利用制备的分子印迹电化学传感器对食品中的沙丁胺醇进行了测定,回收率在86.5%~111.9%(n=3),结果令人满意。
[Abstract]:The surface of glassy carbon electrode was modified by electrodeposition of nano-gold and electropolymerization of polythionine, and then 伪 -methacrylic acid was electropolymerized by using salbutamol as template molecule. A selective gold / polythionine / salbutamol molecularly imprinted electrochemical sensor was prepared. Different modified electrodes were characterized by cyclic voltammetry and differential pulse method. The preparation and determination conditions of the electrodes were optimized. The selective response of the imprinting sensors to template molecules was studied. The results showed that under the optimum preparation conditions, the template molecule consisted of functional monomer 1: 4, polymerized for 15 cycles, eluted with 0.5 mmol / L sulfuric acid and acetonitrile (1: 9 V / V) for 10 min, and DPV was detected in PBS solution of pH 7.0. The linear range was 2.0 脳 10-71.0 脳 10-4 mol / L, and the detection limit was 6.0 脳 10-8 mol / L / N ~ (-1). The sensor has high sensitivity, strong anti-interference ability, good reproducibility and stability. Salbutamol in food was determined by molecularly imprinted electrochemical sensor. The recovery of salbutamol in food was 86.5% (n ~ (3). The results were satisfactory.
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