正交试验结合HPLC指纹图谱优选丹参总酚酸的提取工艺

发布时间：2018-07-02 23:28

本文选题：丹参总酚酸提取物 + HPLC特征图谱　；参考：《中药材》2017年05期

【摘要】：目的:优选丹参中丹参总酚酸的最佳提取工艺。方法:采用HPLC指纹图谱技术,以5个成分(丹参素、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸B)的含量、丹参总酚酸提取物干浸膏得率及丹参总酚酸提取物的HPLC特征图谱的共有峰总面积之和为综合评判指标,通过L_9(3~4)正交试验设计来考察料液比、提取时间、醇沉溶液的含醇量等因素对丹参总酚酸提取物提取工艺的影响。结果:最佳提取工艺是料液比为1∶12(g/m L)、提取时间为1.5 h、醇沉溶液的含醇量为70%。结论:该实验建立了丹参总酚酸提取物的最佳提取工艺,补充药典中关于丹参总酚酸提取物提取条件的空缺,为以后丹参总酚酸提取物的进一步研究提供了依据。
[Abstract]:Objective: to optimize the extraction process of total phenolic acid from Salvia miltiorrhiza. Methods: the contents of five components (Danshensu, protocatechualdehyde, caffeic acid, rosemary acid, Salvianolic acid B) were determined by HPLC. The dry extract yield of total phenolic acid extract of Salvia miltiorrhiza and the sum of the total common peak area of the HPLC characteristic map of total phenolic acid extract of Salvia miltiorrhiza were comprehensive evaluation indexes. The ratio of material to liquid and extraction time were investigated by L9 (3N4) orthogonal design. Effects of alcohol content of alcohol precipitation solution on extraction process of total phenolic acid extract from Salvia miltiorrhiza. Results: the optimum extraction conditions were as follows: the ratio of material to liquid was 1:12 (g / mL), the extraction time was 1.5 h, and the alcohol content of alcohol precipitated solution was 70%. Conclusion: this experiment established the best extraction technology of total phenolic acid extract of Salvia miltiorrhiza and supplemented the vacancy in pharmacopoeia about the extraction condition of total phenolic acid extract of salvia miltiorrhiza, which provided the basis for further study of total phenolic acid extract of salvia miltiorrhiza.
【作者单位】：安徽中医药大学/安徽中医药大学药物代谢研究所;
【基金】：安徽省科技厅项目(1501ld04004) 安徽省教育厅项目(皖教秘科[2015]49号);安徽省教育厅安徽省财政厅项目(皖教科[2013]2号) 国家中医药管理局项目(国中医药办规财发[2013]45号)
【分类号】：O657.72;R284.1

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