基于光电传感原理的探针构建及其分析检测应用

发布时间：2018-07-02 23:40

本文选题：电化学发光 + 荧光探针　；参考：《上海师范大学》2016年硕士论文

【摘要】：电化学发光的分析技术不仅拥有化学发光的优势,还有较高的灵敏度、测定范围广泛、操作容易、快速以及自动化程度高等优势,已经备受瞩目,而且在免疫分析、环境分析、农残分析、食品分析、药物分析和其他重要基因疾病的分子水平诊断等方面有着良好的发展前景。纳米材料具有大的比表面积、化学反应活性和尺寸效应,已被广泛用于固态电化学发光传感器的构建。在电极表面固定的大量生物分子和发光探针三联吡啶钌,能够显著地提高电化学发光传感器的性能。荧光技术的优点已广为人知,如无创性、高选择性,并且在环境检测、生命科学和疾病诊断方面已经被公认为一种有效的、通用的手段。本研究是将二氧化钛包裹金纳米粒子修饰高岭土的电化学发光传感器的制备及其性质表征;设计一个基于ESIPT原理高选择性的比值型探针及其对水溶液和气体状态下肼NH_2NH_2的方便、快速检测。主要研究内容如下:(1)成功制备出一种廉价、简便的Ru(bpy)32+/Au NPs/HNTs/TiO_2/Nafion电化学发光传感器,并对其进行了一系列性质表征。该传感器的构建方法简单,能够对三丙胺TPA的检测显示出了良好的电化学发光性能,并且能够很好地将电化学发光信号放大,充分体现了Au NPs/HNTs/TiO_2复合材料的优越性及其在该传感器中的信号放大的重要作用。(2)以苯并噻唑类化合物(HMBT)为荧光团母体,设计合成了一种基于ESIPT原理的荧光探针(EPH)。该探针受p H影响较小,性质较为稳定,灵敏度高。当EPH与NH_2NH_2反应后,最大发射波长由371 nm红移到了480 nm。设计的探针EPH对NH_2NH_2有着高度的选择性,可以实现对水溶液和气体状态下肼NH_2NH_2的方便、快速检测。该试纸条由于方便携带、制作成本较低,同时兼具高效和快速检测特点,可用于机场、水质和大气中高浓度NH_2NH_2的监测。
[Abstract]:Electrochemiluminescence analysis technology not only has the advantage of chemiluminescence, but also has high sensitivity, wide range of measurement, easy operation, high speed and high automation, and has attracted much attention in immunoassay and environmental analysis. Agricultural disability analysis, food analysis, drug analysis and molecular level diagnosis of other important genetic diseases have good prospects. Nanomaterials with large specific surface area, chemical reaction activity and size effect have been widely used in the construction of solid-state electrochemiluminescence sensors. A large number of biomolecules fixed on the electrode surface and luminescent probe tripyridine ruthenium can significantly improve the performance of the electroluminescent sensor. The advantages of fluorescence technology have been widely known, such as non-invasive, highly selective, and has been recognized as an effective and universal means in environmental detection, life sciences and disease diagnosis. In this study, the electrochemical luminescent sensor coated with gold nanoparticles was prepared and characterized, a ratio probe based on ESIPT principle was designed and its convenience to NH _ 2NH _ 2 in aqueous and gas state was studied. Rapid detection. The main contents are as follows: (1) A cheap and simple Ru (bpy) 32 / au NPs / HNTs / TIO _ 2 / Nafion electroluminescent sensor has been successfully fabricated and characterized. The method of constructing the sensor is simple, it can show good electroluminescence performance for TPA detection, and it can amplify the electroluminescent signal well. The advantages of au NPs / HNTs / TiO2 composites and their important role in signal amplification in the sensor are fully demonstrated. (2) A fluorescence probe (EPH) based on the principle of ESIPT is designed and synthesized with benzothiazole compounds (HMBT) as the fluorescence cluster matrix. The probe is less affected by pH, stable in nature and high in sensitivity. When EPH reacts with NH2NH2, the maximum emission wavelength shifts from 371 nm to 480 nm red. The designed probe EPH has a high selectivity to NH2NH2 and can be used to detect NH _ 2NH _ 2 in water and gas state conveniently and quickly. The strip is easy to carry, low cost and high efficiency and fast detection. It can be used to monitor the high concentration NH _ 2NH _ 2 in airport, water quality and atmosphere.
【学位授予单位】：上海师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.1

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