超高效液相色谱-串联质谱测定动物肌肉组织中32种β-激动剂、β-阻滞剂和糖肽类抗生素药物残留

发布时间：2018-07-03 20:57

本文选题：β-激动剂 + β-阻滞剂　；参考：《分析化学》2016年02期

【摘要】：建立了同时测定动物肌肉组织中27种β-激动剂、3种β-阻滞剂和2种糖肽类抗生素的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。样品经酶解、蛋白沉淀后,以乙酸乙酯-异丙醇(6∶4,V/V)提取,StarataX-C固相萃取净化,BEH C18色谱柱分离,乙腈-0.1%甲酸梯度洗脱,串联质谱ESI+电离,多反应监测模式检测,外标法定量。结果表明,32种目标物在线性范围内相关性良好(R20.995),多巴胺、瑞普特罗、万古霉素和去甲万古霉素的方法检出限为5μg/kg,其它目标物的检出限为3μg/kg;平均加标回收率为83.6%~103%,相对标准偏差(n=6)为3.9%~10.4%,日间精密度为4.5%~9.8%。结果表明,本方法准确、灵敏,适用于动物肌肉组织中β-激动剂、β-阻滞剂,以及糖肽类抗生素的高通量测定。
[Abstract]:A high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method was developed for simultaneous determination of 27 尾 -agonists, 3 尾 -blockers and 2 glycopeptide antibiotics in animal muscle tissue. After enzymatic hydrolysis and protein precipitation, Starata X-C solid phase extraction (SPE) was used to purify the samples on the chromatographic column, acetonitrile-0.1% formic acid gradient elution, tandem mass spectrometry ESI ionization, multi-reaction monitoring mode detection and external standard method. The results showed that the linear range of 32 target compounds was good (R20.995), dopamine, rapterol, The detection limit of vancomycin and norvancomycin is 5 渭 g / kg, the detection limit of other target compounds is 3 渭 g / kg, the average recovery is 83.6% and 103%, the relative standard deviation (nn ~ (6) is 3.9g / kg and the precision during day is 4.59.8%. The results showed that the method was accurate and sensitive and was suitable for the high-throughput determination of 尾 -agonists, 尾 -blockers and glycopeptide antibiotics in animal muscle tissues.
【作者单位】：广州质量监督检测研究院国家加工食品质量监督检验中心(广州)广州市食品安全检测技术重点实验室广州市食品安全风险动态监测与预警研究中心;
【基金】：国家科技部港澳台科技合作专项基金资助(No.2013DFH30070)~~
【分类号】：TS255.7;O657.63

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