半导体纳米粒子SERS基底对大肠杆菌的无损检测研究

发布时间：2018-07-07 07:21

本文选题：半导体纳米粒子 + 细菌　；参考：《光谱学与光谱分析》2017年05期

【摘要】：很多致命的疾病都与细菌感染密切相关,快速、准确地检测和鉴定细菌及微生物,一直是微生物学家及有关科研工作者追求的目标,拉曼光谱可以提供丰富的谱图信息,而表面增强拉曼光谱(SERS)有很高的检测灵敏度,然而一些贵金属SERS基底却容易使蛋白质变性,影响检测结果。以大肠杆菌(E.Coli)作为目标检测细菌,首先检测到大肠杆菌的拉曼光谱,之后采用两种不同的SERS基底(ZnO,Ag溶胶)进行检测。结果表明Ag溶胶基底有很强且较丰富的SERS信号,但是相对于E.Coli的本体拉曼谱峰有较大位移,说明与银溶胶相互作用的细菌存在一定的蛋白质变性过程;而ZnO纳米粒子与细菌作用的SERS信号虽然较弱,但是与E.Coli的本体拉曼信号较为相似,说明ZnO纳米粒子对E.Coli本体基本无损,这将有利于SERS在生物体系的无损检测。该结果可以为利用生物相容性好的半导体SERS基底进行细菌的检测提供有益的参考。
[Abstract]:Many fatal diseases are closely related to bacterial infection. Rapid and accurate detection and identification of bacteria and microbes have been pursued by microbiologists and researchers concerned. Raman spectroscopy can provide rich spectral information. Surface enhanced Raman spectroscopy (SERS) has high detection sensitivity, however, some noble metals SERS substrates are prone to denaturation of proteins, which affects the detection results. Escherichia coli (E. Coli) was used as the target to detect bacteria. The Raman spectra of E. Coli were first detected and then detected by two different SERS substrates (ZnO- Ag sol). The results show that the Ag sol substrate has strong and abundant SERS signals, but there is a large shift in the bulk Raman spectra of E. Coli, which indicates that the bacteria interacting with the Ag sol have a certain protein denaturation process. Although the SERS signal of the interaction between ZnO nanoparticles and bacteria is weak, it is similar to the bulk Raman signal of E. Coli, which indicates that ZnO nanoparticles are basically nondestructive to E. Coli bulk, which will be beneficial to the nondestructive detection of SERS in biological system. The results can provide a useful reference for the detection of bacteria on a biocompatible semiconductor SERS substrate.
【作者单位】：吉林大学超分子结构与材料国家重点实验室;吉林大学中日联谊医院;
【基金】：国家自然科学基金项目(20903044,21327803,21473068) 吉林省科技发展项目(20140414005GH)资助
【分类号】：O657.37;TS207.4

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