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《荧光探针》专辑序言——荧光探针:点亮生命未知

发布时间:2018-07-10 09:51

  本文选题:荧光探针 + 荧光基团 ; 参考:《应用化学》2017年12期


【摘要】:正荧光技术已发展成为分子生物学、细胞生物学、分析化学、环境检测和临床诊断的有力工具,具有灵敏度高,选择性好,时空分辨率高,设备简单廉价等突出优势。荧光探针通常由荧光基团和识别基团组成。识别基团可以选择性感知环境参数,也可以识别小分子、离子、酶、蛋白、核酸等。识别事件如何产生荧光响应,需要研究荧光发射的基本规律。当染料吸收光到达激发态后,其能量可以荧光形式释放,也可以被内转换、电子转移、能量转移、或系间穿越等过程耗散。识别事件通过对上述激发态能量释放
[Abstract]:Positive fluorescence technology has been developed into a powerful tool for molecular biology, cell biology, analytical chemistry, environmental detection and clinical diagnosis. It has the advantages of high sensitivity, good selectivity, high space-time resolution, simple and cheap equipment and so on. Fluorescence probes usually consist of fluorescent groups and recognition groups. Recognition groups can choose sexy environmental parameters, but also recognize small molecules, ions, enzymes, proteins, nucleic acids and so on. The basic rules of fluorescence emission need to be studied to identify how the event produces fluorescence response. When the dye absorbs light to the excited state, its energy can be released in the form of fluorescence or dissipated by internal conversion, electron transfer, energy transfer, or intersystem crossing. Identify the event by releasing the energy of the excited state
【作者单位】: 大连理工大学精细化工国家重点实验室;
【分类号】:O657.3


本文编号:2113000

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