包裹BSA的线状银为基底探讨小檗碱的拉曼光谱
发布时间：2018-07-15 13:27
【摘要】：通过拉曼光谱技术测定了液体小檗碱(Berberine)的常规拉曼光谱(NRS)及表面增强拉曼光谱(SERS),归属了拉曼特征峰,并与固体小檗碱的NRS进行对比,发现特征峰位基本没变.研究小檗碱与纳米银(Ag-Berberine)、包裹牛血清白蛋白(BSA)的线状银(Ag@BSA-Berberine)的拉曼光谱,分析了线性纳米银(Ag)及包裹BSA的线状银(Ag@BSA)对小檗碱的吸附模型.结果显示,线性纳米银对小檗碱、牛血清白蛋白有明显的拉曼增强效应,增强效应主要在线性纳米银与氨基酸中N的孤电子对、甲氧基中的氧、苯环中的π电子及异喹啉中的N发生了吸附作用.对比Ag@BSA-Berberine和Ag-Berberine的特征峰,加了BSA后的拉曼光谱有明显的减弱,但峰位并没有移动,但是有BSA修饰的纳米银的小檗碱拉曼光谱有较高的稳定性,利于小檗碱的进一步研究.
[Abstract]:The Raman spectroscopy (NRS) and surface enhanced Raman spectroscopy (SERS) were used to determine the Raman spectrum (SERS) of the liquid berberine (Berberine). The Raman spectrum was attributed to the Raman spectrum, and compared with the NRS of the solid berberine, it was found that the characteristics of the berberine were basically unchanged. The linear silver of the berberine and nanomilb (Ag-Berberine) and the bovine serum albumin (BSA) was studied. The adsorption model of Berberine by linear silver (Ag) and linear silver (Ag@BSA) wrapped in BSA was analyzed by Raman spectroscopy. The results showed that the linear nano silver had obvious Raman enhancement effect on berberine and bovine serum albumin, and the enhancement effect was mainly on the on-line nano silver and the N in the amino acid and the oxygen in the methoxy group, and the effect of the linear nano silver on the berberine and bovine serum albumin (BSA) was obviously enhanced. The adsorption of N in the pion and isoquinoline in the benzene ring has been adsorbed. Compared with the characteristic peaks of Ag@BSA-Berberine and Ag-Berberine, the Raman spectra after adding BSA are obviously weakened, but the peak position does not move, but the Raman spectra of the berberine with BSA modified nano silver have high stability, which is beneficial to further study of berberine.
【作者单位】： 西南大学化学化工学院;
【基金】：国家自然科学基金(21277110)
【分类号】：TQ464.4;O657.37
，
本文编号：2124226
[Abstract]:The Raman spectroscopy (NRS) and surface enhanced Raman spectroscopy (SERS) were used to determine the Raman spectrum (SERS) of the liquid berberine (Berberine). The Raman spectrum was attributed to the Raman spectrum, and compared with the NRS of the solid berberine, it was found that the characteristics of the berberine were basically unchanged. The linear silver of the berberine and nanomilb (Ag-Berberine) and the bovine serum albumin (BSA) was studied. The adsorption model of Berberine by linear silver (Ag) and linear silver (Ag@BSA) wrapped in BSA was analyzed by Raman spectroscopy. The results showed that the linear nano silver had obvious Raman enhancement effect on berberine and bovine serum albumin, and the enhancement effect was mainly on the on-line nano silver and the N in the amino acid and the oxygen in the methoxy group, and the effect of the linear nano silver on the berberine and bovine serum albumin (BSA) was obviously enhanced. The adsorption of N in the pion and isoquinoline in the benzene ring has been adsorbed. Compared with the characteristic peaks of Ag@BSA-Berberine and Ag-Berberine, the Raman spectra after adding BSA are obviously weakened, but the peak position does not move, but the Raman spectra of the berberine with BSA modified nano silver have high stability, which is beneficial to further study of berberine.
【作者单位】： 西南大学化学化工学院;
【基金】：国家自然科学基金(21277110)
【分类号】：TQ464.4;O657.37
，
本文编号：2124226
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