荧光碳点的制备、材料化及其性能研究

发布时间：2018-07-23 11:32

【摘要】：荧光碳点是继富勒烯、碳纳米管及石墨烯之后最热门的碳纳米材料之一。这种纳米材料克服了传统量子点的某些缺点,不仅具有优良的光学性能与小尺寸特性,而且具有良好的生物相容性,易于实现表面功能化,在生化传感、成像分析、环境检测、光催化技术及药物载体等领域具有很好的应用潜力。本文主要从荧光碳点的制备与应用两个方面出发,首先,用柠檬酸和氨基化合物制备了具有高荧光量子产率和其他优异荧光性能的碳点;其次,将表面官能化的荧光碳点应用于金属离子及小分子的检测；最后,将小分子的罗丹明类荧光探针连接到碳点表面,设计合成了荧光探针修饰的功能材料。论文的主要内容概括如下：1 分别使用柠檬酸与1,2-乙二胺(NCD)和N-氨乙基-3-氨基丙基甲基二甲氧基硅烷(SiCD)制备了两种碳点。NCD和SiCD的量子产率分别高达65.5%和55.4%。NCD和SiCD的荧光均可在水相中被痕量的Hg2+猝灭,在此基础上开发出两种具有高选择性和灵敏性的Hg2+荧光探针。两种探针被成功用于Hg2+在细胞中的成像。2首次研究了硅烷官能化SiCD和槲皮素之间的荧光相互作用,并发现SiCD可以高选择性和灵敏性地通过荧光和紫外吸收检测槲皮素。在此基础上实现槲皮素在水相中的快速检测,碳点的荧光强度与槲皮素浓度的线性范围为0-40μM,最低检测限为79 nm。3用柠檬酸与二乙烯三胺制备了碳点N2CD,其量子产率高达86%。通过N2CD和槲皮素构建一个基于荧光共振能量转移(FRET)的比率型荧光检测系统,实现对A1~(3+)的检测。在FRET比率型荧光检测系统中,N2CD为供体,槲皮素-A1~(3+)络合物作为受体。此外,基于N2CD/QCT-Al~(3+)的FRET比率型荧光系统被制作成荧光检测试纸,通过色度分析实现对A1~(3+)的检测。4合成了基于罗丹明-三聚氯氰的A1~(3+)荧光探针Rh-1,可通过荧光和颜色高选择性地检测A1~(3+)。通过N2CD表面的氨基,将其固载到N2CD上,设计合成了一种荧光检测平台Rh-CD。Rh-CD不仅保持的Rh-1的检测性能,而且具有优良的水溶性和生物相容性。
[Abstract]:Fluorescent carbon spot is one of the most popular carbon nanomaterials after fullerene, carbon nanotubes and graphene. This nanometer material overcomes some shortcomings of traditional quantum dots, not only has excellent optical properties and small size characteristics, but also has good biocompatibility, easy to realize surface functionalization, in biochemical sensing, imaging analysis, environmental detection, Photocatalytic technology and drug carrier have good application potential. In this paper, we mainly focus on the preparation and application of fluorescent carbon points. Firstly, we have prepared carbon points with high fluorescence quantum yield and other excellent fluorescence properties using citric acid and amino compounds. The surface functionalized fluorescent carbon spots were applied to the detection of metal ions and small molecules. Finally, the Rhodamine fluorescent probes of small molecules were connected to the carbon point surface, and the functional materials modified by fluorescent probes were designed and synthesized. The main contents of the thesis are summarized as follows: 1. The quantum yields of the two carbon dots. NCD and SiCD were respectively 65.5% and 55.4b. NCD and SiCD were prepared by using citric acid, 1nd-ethylenediamine (NCD) and N-aminoethyl-3-aminopropylmethyldimethoxy silane (SiCD), respectively. Fluorescence can be quenched by trace Hg _ 2 in aqueous phase. On this basis, two Hg2 fluorescent probes with high selectivity and sensitivity were developed. The fluorescence interaction between silane functionalized SiCD and quercetin was studied for the first time by using two probes in Hg2 imaging 2.It was found that SiCD could detect quercetin by fluorescence and UV absorption with high selectivity and sensitivity. On this basis, the rapid detection of quercetin in aqueous phase was realized. The linear range of fluorescence intensity of carbon spot and the concentration of quercetin was 0-40 渭 m, and the lowest detection limit was 79 nm.3. The carbon dot N _ 2CD was prepared by citric acid and diethylenetriamine. The quantum yield was up to 86%. A ratio fluorescence detection system based on fluorescence resonance energy transfer (FRET) was constructed by N2CD and quercetin to detect A1 ~ (3). In the fret ratio fluorescence detection system, N2CD was used as donor, quercetin A 1 ~ (3) complex was used as receptor. In addition, the FRET ratio fluorescence system based on N2CD / QCT-Al3 was made into a fluorescent test paper. The fluorescence probe Rh-1 based on rhodamine-melamine cyanide was synthesized by colorimetric analysis to detect A1 ~ (3), which can be detected selectively by fluorescence and color. A fluorescence detection platform Rh-CD.Rh-CD was designed and synthesized by immobilization of amino group on N2CD surface to N2CD. Rh-CD.Rh-CD not only maintains the detection performance of Rh-1, but also has excellent water solubility and biocompatibility.
【学位授予单位】：天津工业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3;O613.71

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