高灵敏表面增强拉曼衬底的研制
发布时间：2018-08-03 09:57
【摘要】：自从表面增强拉曼散射(SERS)被发现以来,SERS光谱技术因其高灵敏、快速、高效、简单的优点被广泛应用于生物、医学、化学分析等领域。特别是当目标分子数量低于ppb级别时,SERS光谱的优势显得尤为突出,它可以在复杂的体系中检测到生物化学类样品的指纹特征拉曼谱,可用于检测、鉴定甚至标定含量。近年来我国的食品安全问题时有发生,简单高效的SERS光谱技术在食品安全检测中发挥了重要的作用。将SERS技术应用于食品安全检测最关键的是制备出经济、增强效果好、可重复性高的SERS衬底。本论文利用FDTD方法对金字塔、截角金字塔、椭球体、方块、米字形和点阵形貌金衬底的散射光谱和空间电场分布进行了理论模拟计算,分析了衬底的表面等离子体共振特性,研究了各自的SERS效果。模拟结果表明将衬底表面微形貌化会产生较好的增强效果,并且复杂的形貌比单一的形貌增强效果更好,同时形貌的尺寸改变时,散射光谱的规律也会发生变化。实验中利用电子束曝光和软模板压印技术成功制备出了米字形和点阵形貌的金衬底,并将其用于胱氨酸和三聚氰胺的拉曼散射光谱的测量,测量结果表明米字形金衬底和点阵金衬底都能检测到浓度为50ppm的胱氨酸的拉曼特征峰,并且米字形金衬底也可以检测到浓度为50ppm的三聚氰胺的拉曼特征峰。
[Abstract]:Since the discovery of surface-enhanced Raman scattering (SERS) spectroscopy technology has been widely used in biological, medical, chemical analysis and other fields because of its high sensitivity, fast, high efficiency and simple advantages. Especially when the number of target molecules is lower than the ppb level, the advantage of SERS spectrum is particularly obvious. It can detect the characteristic Raman spectrum of biochemistry samples in complex system, and can be used to detect, identify and even calibrate the content. In recent years, food safety problems occur from time to time in our country. Simple and efficient SERS spectrum technology plays an important role in food safety detection. The key to the application of SERS in food safety detection is to prepare SERS substrates which are economical, effective and reproducible. In this paper, the scattering spectra and spatial electric field distribution of gold substrates with pyramid, truncated pyramid, ellipsoid, block, meter shape and lattice topography are simulated by FDTD method. The surface plasmon resonance characteristics of the substrate are analyzed. The effects of SERS were studied. The simulation results show that the microtopography of the substrate surface will produce a better enhancement effect, and the complex morphology is better than the single morphology enhancement effect. At the same time, when the size of the morphology changes, the law of the scattering spectrum will also change. In the experiment, the gold substrates with meter shape and lattice morphology were successfully prepared by electron beam exposure and soft template imprint techniques, and used to measure the Raman scattering spectra of cystine and melamine. The results show that the Raman characteristic peaks of cystine at the concentration of 50ppm can be detected on the meter shaped gold substrate and the lattice gold substrate, and the Raman characteristic peak of melamine at the concentration of 50ppm can also be detected on the meter shaped gold substrate.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.37
本文编号：2161383
[Abstract]:Since the discovery of surface-enhanced Raman scattering (SERS) spectroscopy technology has been widely used in biological, medical, chemical analysis and other fields because of its high sensitivity, fast, high efficiency and simple advantages. Especially when the number of target molecules is lower than the ppb level, the advantage of SERS spectrum is particularly obvious. It can detect the characteristic Raman spectrum of biochemistry samples in complex system, and can be used to detect, identify and even calibrate the content. In recent years, food safety problems occur from time to time in our country. Simple and efficient SERS spectrum technology plays an important role in food safety detection. The key to the application of SERS in food safety detection is to prepare SERS substrates which are economical, effective and reproducible. In this paper, the scattering spectra and spatial electric field distribution of gold substrates with pyramid, truncated pyramid, ellipsoid, block, meter shape and lattice topography are simulated by FDTD method. The surface plasmon resonance characteristics of the substrate are analyzed. The effects of SERS were studied. The simulation results show that the microtopography of the substrate surface will produce a better enhancement effect, and the complex morphology is better than the single morphology enhancement effect. At the same time, when the size of the morphology changes, the law of the scattering spectrum will also change. In the experiment, the gold substrates with meter shape and lattice morphology were successfully prepared by electron beam exposure and soft template imprint techniques, and used to measure the Raman scattering spectra of cystine and melamine. The results show that the Raman characteristic peaks of cystine at the concentration of 50ppm can be detected on the meter shaped gold substrate and the lattice gold substrate, and the Raman characteristic peak of melamine at the concentration of 50ppm can also be detected on the meter shaped gold substrate.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.37
【参考文献】
相关期刊论文 前10条
1 孙美娟;陶站华;;表面增强拉曼光谱技术在生物学中的应用[J];广西师范学院学报(自然科学版);2014年04期
2 汤建;刘爱萍;李培刚;沈静琴;唐为华;;界面自组装的金/氧化石墨烯复合材料的表面增强拉曼散射行为研究[J];物理学报;2014年10期
3 王东;刘红缨;贺军辉;刘林林;;旋涂法制备功能薄膜的研究进展[J];影像科学与光化学;2012年02期
4 赵彬;张静;周伟民;王金合;刘彦伯;张燕萍;施利毅;张剑平;;纳米压印光刻胶[J];微纳电子技术;2011年09期
5 陈彰旭;郑炳云;李先学;傅明连;谢署光;邓超;胡衍华;;模板法制备纳米材料研究进展[J];化工进展;2010年01期
6 刘文涵;杨未;张丹;;苯丙氨酸银溶胶表面增强拉曼光谱的研究[J];光谱学与光谱分析;2008年02期
7 高书燕;张树霞;杨恕霞;张洪杰;;表面增强拉曼散射活性基底[J];化学通报;2007年12期
8 刘欢;翟锦;江雷;;纳米材料的自组装研究进展[J];无机化学学报;2006年04期
9 周增会,王桂英,徐至展;表面增强拉曼光谱术在生命科学及单分子研究中的应用与进展[J];激光与光电子学进展;2005年10期
10 王莉,卢秉恒,崔东印,丁玉成,刘红忠;多层冷压印光刻中超高精度对正的研究[J];西安交通大学学报;2004年09期
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