氨基酸改性壳聚糖抗菌材料制备及其性能研究

发布时间：2018-08-12 07:52

【摘要】：以壳聚糖(CS)为基体,在缩合剂1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC·HCl),偶联剂N-羟基-丁二酰亚胺(NHS)的存在下,将L-精氨酸接枝到CS大分子上,制备出一种天然高分子基重金属螯合及抗菌材料,L-精氨酸接枝壳聚糖CS-L-Arginine(CA)。阐述了反应机理,用采用元素分析、拉曼光谱(RAM)、核磁共振谱(13C NMR)以及X射线衍射谱(XRD)分析与表征了产物的结构产物。加入山梨醇作为增塑剂,采用涂膜法制备氨基酸改性壳聚糖膜。实验表明,随着接枝率的提高,改性壳聚糖膜对Ni~(2+)、Cu~(2+)的饱和吸附量的变化趋势先增大再减小,当接枝率为16.85%的改性壳聚糖膜对Ni~(2+)、Cu~(2+)的饱和吸附量达到最优,分别为148.32与198.78mg/g;对大肠杆菌和金黄色葡萄球菌的抑制作用逐渐提高,当接枝率为16.85%时,抑菌效率最佳,可分别达到了92.06%,93.58%。
[Abstract]:Using chitosan (CS) as matrix, L-arginine was grafted onto CS macromolecule in the presence of 1- (3-dimethylaminopropyl) -3-ethyl carbodiimide (EDC HCl), coupling agent, N-hydroxy-succinimide (NHS). Preparation of a Natural Polymer-based heavy Metal chelation and Antibacterial material L- arginine grafted chitosan CS-L-Arginine (CA). The reaction mechanism was described. The structure of the product was characterized by elemental analysis, Raman (RAM), NMR (13C NMR) and X-ray diffraction (XRD). Adding sorbitol as plasticizer, amino acid modified chitosan membrane was prepared by coating method. The experimental results show that with the increase of grafting ratio, the saturated adsorption capacity of modified chitosan membrane for Ni ~ (2) and Cu ~ (2) increases first and then decreases. When the grafting ratio is 16.85%, the saturated adsorption capacity of modified chitosan membrane for Ni ~ (2) and Cu ~ (2) is optimal. The inhibitory effect on Escherichia coli and Staphylococcus aureus was increased gradually, and when the grafting rate was 16.85mg / g, the bacteriostatic efficiency was the best, reaching 92.06g / g and 93.58 respectively.
【作者单位】：天津工业大学纺织学院;
【基金】：国家级现代农业产业技术体系专项资助项目(CARS-44-D-2) 国家自然科学基金资助项目(51503147)
【分类号】：O636.1

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