基于氧化石墨烯的AIE荧光探针对肝素的检测

发布时间：2018-08-30 13:25

【摘要】：合成了一种新的聚集诱导发光物质,将其负载于氧化石墨烯上制备成新型荧光探针对肝素进行检测。通过改进的维蒂希反应(Wittig reaction)合成3种二苯乙烯类化合物,分别为含有羟基、卤素基团(溴)烷基链和氨基正离子侧链的有机物,并进行核磁表征,结果表明,经过提纯后均得到了纯净的产品。通过荧光发射光谱进行表征,确定了这3种化合物的聚集诱导发光特性。将其中含有氨基正离子侧链的化合物负载于氧化石墨烯,利用这种荧光探针对肝素进行检测。结果表明,氧化石墨烯具有荧光淬灭作用,与带有正电荷的聚集诱导发光物质通过静电作用复合,使其表面修饰正电,荧光淬灭。当加入肝素钠后,由于肝素钠电负性更强,使得两者脱离,荧光显著增强,从而可通过此方法对肝素进行检测。
[Abstract]:A new aggregation induced luminescent substance was synthesized and loaded on graphene oxide to prepare a new fluorescent probe for the detection of heparin. Three kinds of stilbene compounds were synthesized by improved Wittig reaction (Wittig reaction). They were organic compounds containing hydroxyl, halogen (bromine) alkyl chain and amino cationic side chain, and were characterized by NMR. After purification, pure products were obtained. The aggregation induced luminescence characteristics of these three compounds were determined by fluorescence emission spectra. The compounds containing side chain of positive amino ions were loaded with graphene oxide, and heparin was detected by this fluorescence probe. The results show that graphene oxide has the effect of fluorescence quenching, which is combined with the aggregation induced luminescent material with positive charge through electrostatic interaction to make its surface modified with positive charge and fluorescence quenched. After the addition of heparin sodium, the more electronegativity of heparin sodium, the fluorescence of heparin can be detected by this method.
【作者单位】：天津大学化工学院;
【基金】：国家自然科学基金青年科学基金(批准号:21506157) 高等学校学科创新引智计划(批准号:B06006)资助的课题
【分类号】：O657.3;TQ464

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