超高效液相色谱-飞行时间-串联质谱法用于大白菜叶片中的脂质成分分析

发布时间：2018-09-06 18:26

【摘要】：建立了超高效液相色谱-飞行时间-串联质谱(UPLC-TOF-MS/MS)分析大白菜叶片中脂质的种类、结构、脂肪酸组成并检测其相对含量的方法。以Acquity UPLCTMBEH C8色谱柱为固定相,采用飞行时间质谱全扫描-信息关联采集-子离子扫描(TOF-MS scan-IDA-product ion scan)复合模式实现一次进样分析,同时获得脂质的一级和二级质谱信息。在大白菜叶片中共鉴定得到232种脂质,包含104种磷脂、63种糖脂和65种甘油酯。其中,磷脂、糖脂和甘油酯中的主要成分分别为磷脂酰胆碱、单半乳糖二酰甘油和甘油二酯、甘油三酯。结果表明,该方法具有灵敏度高、准确度高和高通量等优点,为植物脂质的代谢研究提供了可靠的分析技术平台,并为进一步的脂质生物学功能研究奠定了基础。
[Abstract]:A high performance liquid chromatography-time-of-flight tandem mass spectrometry (UPLC-TOF-MS/MS) method was established for the determination of lipid species, structure, fatty acid composition and relative content in Chinese cabbage leaves. The Acquity UPLCTMBEH C8 column was used as the stationary phase, and the first-order and second-order mass spectrometry information of lipids was obtained by using the time-of-flight mass spectrometry (TMS) full scanning, information association acquisition (TOF-MS scan-IDA-product ion scan) and subion scanning (TOF-MS scan-IDA-product ion scan). A total of 232 kinds of lipids were identified in leaves of Chinese cabbage, including 104 kinds of phospholipids, 63 kinds of sugar and lipids, and 65 kinds of glycerides. The main components of phospholipids, glycolipid and glycerides were phosphatidylcholine, monogalactosylglycerol, diglyceride and triglyceride, respectively. The results show that this method has the advantages of high sensitivity, high accuracy and high throughput. It provides a reliable analytical platform for the study of plant lipid metabolism, and lays a foundation for the further study of lipid biological function.
【作者单位】：中国农业科学院蔬菜花卉研究所农业部蔬菜产品质量安全风险评估实验室(北京)农业部园艺作物生物学与种质创制重点实验室;
【基金】：国家蔬菜产品质量安全风险评估项目(GJFP201601401) 食用农产品特征性营养组成识别与验证评估项目(GJFP2016015) 蔬菜产品未知危害因子识别与已知危害因子安全性评估项目(GJFP2016002)~~
【分类号】：S634.1;O657.63

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