密闭微波辅助提取-HPLC法测定还亮草中硬飞燕草碱和巴比翠雀碱的含量
发布时间：2018-09-07 07:02
【摘要】：采用密闭微波辅助法(PMAE)提取还亮草中的硬飞燕草碱和巴比翠雀碱。采用单因素试验结合正交试验方法对微波实验条件进行优化。得到硬飞燕草碱和巴比翠雀碱的最佳提取方案:药物颗粒度100目,固液比1∶60,微波温度80℃,微波功率560 W,微波时间10 min。以甲醇-0.2%三乙胺(45∶55)为流动相,建立了高效液相色谱(HPLC)测定硬飞燕草碱和巴比翠雀碱含量的方法。硬飞燕草碱和巴比翠雀碱分别在0.50~50.0,0.30~30.0 mg/L范围内呈良好的线性关系,回收率为98.3%~104.5%,相对标准偏差(RSD)分别为2.0%和2.2%。与传统溶剂回流法(SRE)进行比较,该方法简单、提取率更高。
[Abstract]:A closed microwave assisted (PMAE) method was used to extract the alkaloids and barbiturine from Hedyotis huanliensis. The condition of microwave experiment was optimized by single factor test and orthogonal test. The optimum extraction scheme of Euphorbiferine and barbitidine was obtained as follows: drug particle size 100 mesh, solid-liquid ratio 1: 60, microwave temperature 80 鈩，
本文编号：2227544
[Abstract]:A closed microwave assisted (PMAE) method was used to extract the alkaloids and barbiturine from Hedyotis huanliensis. The condition of microwave experiment was optimized by single factor test and orthogonal test. The optimum extraction scheme of Euphorbiferine and barbitidine was obtained as follows: drug particle size 100 mesh, solid-liquid ratio 1: 60, microwave temperature 80 鈩，
本文编号：2227544
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