超高效液相色谱-串联质谱法检测鱼肉和暂养水中丁香酚残留量
发布时间：2018-09-10 17:55
【摘要】：建立超高效液相色谱-串联质谱测定鱼肉和暂养水中丁香酚残留量的分析方法。鱼肉样品经正己烷提取,水样则直接用HLB小柱浓缩、富集与净化,以电喷雾离子源负离子模式,在多反应监测模式下测定,内标法定量。结果表明,样品在4 min内完成测定,在1~200μg/L质量浓度范围内,相关系数(R~2)大于0.996,鱼肉样品的加标回收率在88.6%~106%之间,相对标准偏差为7.82%~11.90%;水样的加标回收率在89.2%~96.5%之间,相对标准偏差为5.18%~7.83%。鱼肉和水样中丁香酚的定量限分别为2.5μg/kg和0.05μg/L。
[Abstract]:A method for the determination of eugenol residues in fish meat and temporary aquaculture water by ultra-high performance liquid chromatography-tandem mass spectrometry was developed.The fish meat samples were extracted by n-hexane and the water samples were concentrated and purified by HLB column.The samples were determined by electrospray ionization under multi-reaction monitoring mode and quantified by internal standard method. The relative standard deviation was 7.82%~11.90%. The relative standard deviation was between 89.2%~96.5%. The relative standard deviation was 5.18%~7.83%. The quantitative limits of eugenol in fish and water samples were 2.5 ug/kg and 2.5 ug/kg, respectively. 0.05 mu g/L.
【作者单位】： 中国水产科学研究院南海水产研究所农业部水产品加工重点实验室农业部水产品贮藏保鲜质量安全风险评估实验室(广州)农业部渔业环境及水产品质量监督检验测试中心(广州);
【基金】：中国水产科学研究院南海水产研究所基本科研业务费项目(2014TS28) 广东省水产品质量安全专项(GDSCZA2015008) 国家农产品质量安全风险评估重大专项(GJFP201501003)
【分类号】：TS254.7;O657.63
本文编号：2235183
[Abstract]:A method for the determination of eugenol residues in fish meat and temporary aquaculture water by ultra-high performance liquid chromatography-tandem mass spectrometry was developed.The fish meat samples were extracted by n-hexane and the water samples were concentrated and purified by HLB column.The samples were determined by electrospray ionization under multi-reaction monitoring mode and quantified by internal standard method. The relative standard deviation was 7.82%~11.90%. The relative standard deviation was between 89.2%~96.5%. The relative standard deviation was 5.18%~7.83%. The quantitative limits of eugenol in fish and water samples were 2.5 ug/kg and 2.5 ug/kg, respectively. 0.05 mu g/L.
【作者单位】： 中国水产科学研究院南海水产研究所农业部水产品加工重点实验室农业部水产品贮藏保鲜质量安全风险评估实验室(广州)农业部渔业环境及水产品质量监督检验测试中心(广州);
【基金】：中国水产科学研究院南海水产研究所基本科研业务费项目(2014TS28) 广东省水产品质量安全专项(GDSCZA2015008) 国家农产品质量安全风险评估重大专项(GJFP201501003)
【分类号】：TS254.7;O657.63
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