不同加工玄参药材中多元功效成分的含量测定及灰色关联度分析
发布时间：2018-09-11 20:58
【摘要】：建立了超高效液相色谱-三重四极杆-线性离子阱质谱(UPLC-Qtrap-MS/MS)同时测定玄参药材中环烯醚萜苷类、苯丙素苷类和有机酸类等12种功效成分的方法,分析了不同加工方法对玄参药材中多元功效成分含量的影响,并采用UPLC-Qtrap-MS/MS技术同时测定其中的12种功效成分含量,用灰色关联度分析法对多元功效成分进行综合评价。结果表明,12种功效成分在一定浓度范围内的线性关系良好,相关系数均大于0.999 0,加标回收率为95.82%~99.35%,相对标准偏差(RSD)小于2.44%;不同加工方法对玄参中多元功效成分含量具有一定的影响,灰色关联度分析结果显示,阴干法和完整药材蒸后烘干的样品综合质量较好。该实验揭示了加工对玄参中多元功效成分的影响,可为优选玄参适宜产地加工方法提供基础资料,同时为玄参药材内在质量的综合评价提供方法参考。
[Abstract]:A high performance liquid chromatography-triplex quadrupole linear ion trap mass spectrometry (UPLC-Qtrap-MS/MS) was developed for the simultaneous determination of 12 components of Cyclene terpene glycosides, phenylallic glycosides and organic acids. The effect of different processing methods on the content of multi-functional components in Radix basalissima was analyzed. The contents of 12 functional components were determined simultaneously by UPLC-Qtrap-MS/MS technique, and the comprehensive evaluation of multi-functional components was carried out by grey correlation analysis. The results showed that there was a good linear relationship among the 12 effective components in a certain concentration range. The correlation coefficient was more than 0.999, the recovery rate was 95.82% and the relative standard deviation (RSD) was less than 2.44. The different processing methods had certain influence on the content of multi-functional components in Radix basalticum. The results of grey correlation analysis showed that the relative standard deviation was less than 2.44. The comprehensive quality of samples dried by Yin-drying method and complete medicinal materials was better. The experiment revealed the effect of processing on the multi-functional components in Radix basaltifolia, which could provide the basic data for the optimum selection of processing methods in the suitable producing areas, and also provide a method reference for the comprehensive evaluation of the internal quality of the medicinal materials of Radix Xuanshen.
【作者单位】： 南京中医药大学;扬州市药品检验所;
【基金】：江苏高校优势学科建设工程项目(ysxk-2014)资助
【分类号】：O657.63;R284
本文编号：2237822
[Abstract]:A high performance liquid chromatography-triplex quadrupole linear ion trap mass spectrometry (UPLC-Qtrap-MS/MS) was developed for the simultaneous determination of 12 components of Cyclene terpene glycosides, phenylallic glycosides and organic acids. The effect of different processing methods on the content of multi-functional components in Radix basalissima was analyzed. The contents of 12 functional components were determined simultaneously by UPLC-Qtrap-MS/MS technique, and the comprehensive evaluation of multi-functional components was carried out by grey correlation analysis. The results showed that there was a good linear relationship among the 12 effective components in a certain concentration range. The correlation coefficient was more than 0.999, the recovery rate was 95.82% and the relative standard deviation (RSD) was less than 2.44. The different processing methods had certain influence on the content of multi-functional components in Radix basalticum. The results of grey correlation analysis showed that the relative standard deviation was less than 2.44. The comprehensive quality of samples dried by Yin-drying method and complete medicinal materials was better. The experiment revealed the effect of processing on the multi-functional components in Radix basaltifolia, which could provide the basic data for the optimum selection of processing methods in the suitable producing areas, and also provide a method reference for the comprehensive evaluation of the internal quality of the medicinal materials of Radix Xuanshen.
【作者单位】： 南京中医药大学;扬州市药品检验所;
【基金】：江苏高校优势学科建设工程项目(ysxk-2014)资助
【分类号】：O657.63;R284
【相似文献】
相关期刊论文 前3条
1 勾中智;徐维盛;刘静;易有金;杨月欣;;脐橙中6种功效成分RP-HPLC检测方法的建立[J];食品研究与开发;2014年05期
2 何康昊;邹晓莉;曾红燕;;11种心血管保健功效成分的反相高效液相色谱测定[J];分析试验室;2014年06期
3 ;[J];;年期
相关硕士学位论文 前1条
1 朱小兰;液相色谱—质谱在保健食品功效成分和违禁药物分析中的应用研究[D];湖南师范大学;2004年
，本文编号：2237822
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2237822.html
教材专著
