含金属卟啉的水溶性共轭聚电解质的设计、合成及其生物应用
发布时间：2018-09-13 07:28
【摘要】：水溶性共轭聚合物(WSCPs)通常由控制其主要光学性质的π共轭主链和控制其水溶性的侧链组成。WSCPs在生物医学方面具有很多优势,如高的亮度、大的消光系数、优异的耐光性、低的细胞毒性和良好的水溶液稳定性。金属卟啉是生命存在的基石之一,在自然界中有独特的生物学功能。它们都被广泛的应用在生物传感,荧光成像,医学和光动力治疗等领域。本文结合WSCPs和金属卟啉两者的优势,合成了一类含金属卟啉的WSCPs材料,研究了其在细胞成像、磁共振成像(MRI)和光动力治疗(PDT)方面的应用。1、通过将具有T1加权成像功能的锰卟啉引入到聚芴基共轭聚合物中,合成了具有荧光/MRI双模式成像功能的水溶性共轭聚电解质。双亲性的化学结构使其可以在PBS缓冲溶液中经过自组装形成100 nm左右粒径均一的球形纳米粒子,其光物理性质经过紫外-可见吸收光谱和荧光光谱进行了表征,形貌通过射电子扫描显微镜(TEM)和动态光散射(DLS)进行了表征。结果表明水溶性共轭聚合物纳米粒子具有强的吸收、高的荧光量子产率、好的稳定性和生物相容性、优异的细胞成像和高的弛豫效率值(r1=13.15 mM-1S-1),这些结果表明其在活细胞成像和MRI方面有很好的应用前景。2、设计合成了一种含有金属钯卟啉的新型共轭聚合物,借助其两亲性的分子结构,在水溶液中经过自组装形成30 nm左右的纳米粒子。利用核磁(NMR)和凝胶渗透色谱(GPC)对中间体和目标聚合物的结构进行了表征。利用TEM和DLS对水溶性共轭聚合物纳米粒子的形貌进行了研究。实验结果表明该聚合物纳米粒子粒径均一,具有良好的分散性和稳定性,可以进行细胞染色。重要的是,水溶性共轭聚合物纳米粒子在光照条件下可以有效地产生单线态氧,使肿瘤细胞凋亡,实现了细胞成像和PDT的目的。
[Abstract]:The water-soluble conjugated polymer (WSCPs) is usually composed of 蟺 conjugated main chains which control its main optical properties and side chains that control its water solubility. WSCPs have many advantages in biomedical fields, such as high brightness, large extinction coefficient, excellent light resistance. Low cytotoxicity and good stability of aqueous solution. Metalloporphyrin is one of the cornerstones of life and has unique biological functions in nature. They are widely used in biosensor, fluorescence imaging, medicine and photodynamic therapy. Combined with the advantages of WSCPs and metalloporphyrin, a class of WSCPs materials containing metalloporphyrin were synthesized, and their cellular imaging was studied. The application of magnetic resonance imaging (MRI) and photodynamic therapy (PDT). 1. By introducing manganese porphyrin with T1 weighted imaging into polyfluorene conjugated polymer, a water-soluble conjugated polyelectrolyte with fluorescence / MRI dual-mode imaging was synthesized. The amphiphilic chemical structure made it possible to self-assemble in PBS buffer solution to form spherical nanoparticles with about 100 nm homogeneous diameter. Their photophysical properties were characterized by UV-Vis absorption and fluorescence spectra. The morphology was characterized by (TEM) and (DLS). The results show that the water-soluble conjugated polymer nanoparticles have strong absorption, high fluorescence quantum yield, good stability and biocompatibility. Excellent cell imaging and high relaxation efficiency (R1C 13.15 mM-1S-1). These results show that it has a good application prospect in living cell imaging and MRI. A novel conjugated polymer containing PD porphyrin was designed and synthesized. With the help of its amphiphilic molecular structure, nanoparticles were self-assembled in aqueous solution for about 30 nm. The structures of intermediates and target polymers were characterized by NMR (NMR) and gel permeation chromatography (GPC). The morphology of water-soluble conjugated polymer nanoparticles was studied by TEM and DLS. The results show that the polymer nanoparticles have uniform particle size, good dispersion and stability, and can be stained by cells. It is important that the water-soluble conjugated polymer nanoparticles can effectively produce singlet oxygen under illumination, which can make tumor cells apoptosis and achieve the purpose of cell imaging and PDT.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O631
本文编号：2240477
[Abstract]:The water-soluble conjugated polymer (WSCPs) is usually composed of 蟺 conjugated main chains which control its main optical properties and side chains that control its water solubility. WSCPs have many advantages in biomedical fields, such as high brightness, large extinction coefficient, excellent light resistance. Low cytotoxicity and good stability of aqueous solution. Metalloporphyrin is one of the cornerstones of life and has unique biological functions in nature. They are widely used in biosensor, fluorescence imaging, medicine and photodynamic therapy. Combined with the advantages of WSCPs and metalloporphyrin, a class of WSCPs materials containing metalloporphyrin were synthesized, and their cellular imaging was studied. The application of magnetic resonance imaging (MRI) and photodynamic therapy (PDT). 1. By introducing manganese porphyrin with T1 weighted imaging into polyfluorene conjugated polymer, a water-soluble conjugated polyelectrolyte with fluorescence / MRI dual-mode imaging was synthesized. The amphiphilic chemical structure made it possible to self-assemble in PBS buffer solution to form spherical nanoparticles with about 100 nm homogeneous diameter. Their photophysical properties were characterized by UV-Vis absorption and fluorescence spectra. The morphology was characterized by (TEM) and (DLS). The results show that the water-soluble conjugated polymer nanoparticles have strong absorption, high fluorescence quantum yield, good stability and biocompatibility. Excellent cell imaging and high relaxation efficiency (R1C 13.15 mM-1S-1). These results show that it has a good application prospect in living cell imaging and MRI. A novel conjugated polymer containing PD porphyrin was designed and synthesized. With the help of its amphiphilic molecular structure, nanoparticles were self-assembled in aqueous solution for about 30 nm. The structures of intermediates and target polymers were characterized by NMR (NMR) and gel permeation chromatography (GPC). The morphology of water-soluble conjugated polymer nanoparticles was studied by TEM and DLS. The results show that the polymer nanoparticles have uniform particle size, good dispersion and stability, and can be stained by cells. It is important that the water-soluble conjugated polymer nanoparticles can effectively produce singlet oxygen under illumination, which can make tumor cells apoptosis and achieve the purpose of cell imaging and PDT.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O631
【参考文献】
相关期刊论文 前1条
1 ;Kumada chain-growth polycondensation as a universal method for synthesis of well-defined conjugated polymers[J];Science China(Chemistry);2010年08期
，本文编号：2240477
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2240477.html
教材专著
