碰撞气体的种类和压力对离子阱质谱性能的影响

发布时间：2018-09-13 07:56

【摘要】：基于数字离子阱技术,研究了离子阱质谱分析实验过程使用的碰撞气体种类及压力对离子阱质谱性能,如质量分辨能力、信号强度、串级质谱分析,以及低质量截止效应等的影响。实验过程中,在离子的激发和碰撞诱导解离阶段,分别采用质量数不等的氦气(质量数=4 amu)、氮气(质量数=28 amu)、氩气(质量数=40 amu)等作为碰撞气体,以及不同的气体压力,研究了它们对质谱性能的影响。结果表明,当采用质量数较大的氩气作为碰撞气体时,可以有效改善低质量数截止效应和提高离子碰撞过程中的能量转移效率,同时提高离子捕获和解离效率,但是质量分辨率会明显降低。在获得较高质量分辨率方面,氦气作为碰撞气体时效果最好。在气压相同的情况下,质量数大的碰撞气体有利于提高串级质谱分析效率,即获得更多碎片离子峰和更多有关母体离子结构的信息。
[Abstract]:Based on the digital ion trap technique, the types of collisional gases used in ion trap mass spectrometry analysis and the performance of pressure on ion trap mass spectrometry, such as mass resolution, signal intensity, cascade mass spectrometry analysis, are studied. And the effect of low quality cutoff effect. In the process of ion excitation and collision induced dissociation, helium (mass number 4 amu), nitrogen) (mass number 28 amu), argon (mass number 40 amu) was used as collision gas, and different gas pressures were used, respectively, in the process of ion excitation and collision-induced dissociation, respectively, with different mass numbers of helium (mass number of 4 amu), nitrogen gas) and mass number of 28 amu), argon (mass number of 40 amu). Their effects on the mass spectrum properties were studied. The results show that the low mass cut-off effect, energy transfer efficiency and ion capture and dissociation efficiency can be improved effectively when argon gas with large mass number is used as collision gas. But the mass resolution is significantly reduced. Helium is the most effective as collision gas in obtaining high mass resolution. Under the same pressure, the collision gas with large mass can improve the efficiency of cascade mass spectrometry, that is to say, more fragment ion peaks and more information about the structure of parent ions can be obtained.
【作者单位】：复旦大学电子工程系;复旦大学化学系激光化学研究所;广州禾信仪器股份有限公司;
【基金】：科技部十二五重大科学仪器设备开发专项(No.2011YQ14015006)资助~~
【分类号】：O657.63

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