电喷雾质谱结合化学计量学方法快速筛选玛咖促睾丸间质细胞增殖活性成分

发布时间：2018-10-04 18:23

【摘要】：应用电喷雾串联质谱法(ESI-MS/MS)对云南和秘鲁产玛咖样品中低极性化学成分进行检测,获得各样品的一级质谱指纹谱图和各离子峰的二级质谱数据,并测定各样品促进大鼠睾丸间质细胞增殖活性,采用化学计量学方法主成分分析(PCA)、偏最小二乘法(PLS)和灰色关联度分析(GRA)对所获得的一级质谱数据进行处理后,可有效区分玛咖的不同产地,且筛选出其中具有促大鼠睾丸间质细胞增殖活性的可能成分,进而通过二级质谱数据分析得到可能活性成分的结构。实验结果表明,玛咖低极性成分具有很好的促大鼠睾丸间质细胞增殖活性,其中活性较强的主要为N-benzylhexadecanamide和N-benzy-(9Z,12Z,15Z)-octadecatrienamide。本方法为简单、快速分析中药中促睾丸间质细胞增殖活性成分筛选方法提供了借鉴。
[Abstract]:The low polar chemical constituents of Maca samples from Yunnan and Peru were detected by electrospray tandem mass spectrometry (ESI-MS/MS). The first order mass spectrometry fingerprint and the secondary mass spectrometry data of each ion peak were obtained. The proliferative activity of rat testis stromal cells was determined. The first order mass spectrometry data were processed by chemometrics principal component analysis (PCA),) partial least square method (PLS) and grey correlation analysis (GRA). It can effectively distinguish the different producing areas of Maca and screen out the possible components which can promote the proliferation of rat testicular interstitial cells and then obtain the structure of the possible active components through the analysis of secondary mass spectrometry data. The results showed that the low polar component of Maca had a good proliferative activity in Leydig cells of rat testis, among which the main ones were N-benzylhexadecanamide and N-benzy- (9ZC12ZN15Z) -octadecatrienamide. This method can be used as a reference for rapid analysis of active components of testicular stromal cell proliferation in traditional Chinese medicine.
【作者单位】：长春中医药大学中医药与生物工程研究开发中心;吉林农业大学中药材学院;长春中医药大学研究生院;
【基金】：国家自然科学基金(No.31570347) 吉林省卫生和计划生育委员会科研计划课题(No.2015Z066)资助~~
【分类号】：O657.63;R285.5

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