拉曼光谱技术在农作物生理信息检测中的研究进展

发布时间：2018-10-13 09:21

【摘要】：拉曼光谱(Raman spectroscopy,RS)是一种散射光谱,具有样品前处理简单、响应速度快、灵敏性高以及原位无损检测等特点。由于拉曼信号具有指纹图谱特性和不受水分信息干扰的优势,其在生物体信息检测方面发挥着重要作用。拉曼光谱成像技术是拉曼光谱技术发展的新方向,其可以同时获取研究对象的空间及光谱信息;显微拉曼光谱技术不仅可以进行分子结构的检测,还能够实现生物组织微区化学成分的空间分布分析。目前,应用拉曼光谱进行农作物生理信息的检测成为学者们的研究热点。本文概述了拉曼光谱的基本原理和分类,并重点介绍了拉曼光谱技术在农作物的生殖与营养器官(种子,花朵,果实和根,茎,叶)中生理信息检测方面的国内外最新研究进展。最后结合国内外研究现状,分析了拉曼光谱在农作物生理信息检测中的局限,并对其的应用前景进行了展望。
[Abstract]:Raman spectroscopy (Raman spectroscopy,RS) is a kind of scattering spectrum, which has the advantages of simple sample pretreatment, fast response, high sensitivity and in situ nondestructive testing. Raman signal plays an important role in the detection of biological information because of its fingerprint characteristics and the advantage of not being interfered by water information. Raman spectroscopy imaging technology is a new direction in the development of Raman spectroscopy technology, it can simultaneously obtain the spatial and spectral information of the object of study, micro-Raman spectroscopy technology can not only be used to detect the molecular structure, It is also possible to analyze the spatial distribution of chemical constituents in biological tissue. At present, the application of Raman spectroscopy in the detection of crop physiological information has become a hot research topic. In this paper, the basic principle and classification of Raman spectroscopy are summarized, and the application of Raman spectroscopy in reproductive and vegetative organs (seeds, flowers, fruits and roots, stems, stems) of crops is emphasized. The latest research progress in physiological information detection at home and abroad. Finally, the limitations of Raman spectroscopy in the detection of crop physiological information are analyzed, and the prospect of its application is prospected.
【作者单位】：浙江大学生物系统工程与食品科学学院;浙江工业大学药学院;西北农林科技大学机械与电子工程学院;
【基金】：国家自然科学基金项目(31471417) 高等学校博士学科点专项科研基金项目(20130101110104)资助
【分类号】：O657.37;S311

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