红花UPLC法特征指纹图谱研究

发布时间：2018-10-22 16:22

【摘要】：目的建立红花Carthamus tinctorius超高效液相色谱(UPLC)指纹图谱,为评价不同产地和不同品种红花的质量提供参考。方法对产地来自新疆和河南共34批红花样品应用UPLC建立其指纹图谱,按"中药指纹图谱相似度评价系统"建立对照图谱,并进行相似度评价。运用SAS 9.30和Matlab软件对新疆不同品种和不同产地红花的UPLC指纹图谱特征峰进行聚类分析和主成分分析。结果建立了红花药材的专属性UPLC指纹图谱,确立了26个共有峰;30批新疆红花样品和4批河南红花样品质量差异较大,新疆不同产地间和同一产地不同品种间具有一定差异。聚类分析和主成分分析均实现了新疆红花指纹图谱差异的识别。结论该方法简便、高效、可靠,为红花药材的质量控制提供了有效的快速评价方法。
[Abstract]:Objective to establish the (UPLC) fingerprint of Carthamus tinctorius for evaluating the quality of safflower in different areas and varieties. Methods A total of 34 batches of safflower samples from Xinjiang and Henan were collected from Xinjiang and Henan. The fingerprints of safflower samples were established by UPLC, and the control map was established according to the similarity evaluation system of traditional Chinese medicine fingerprint, and the similarity was evaluated. Using SAS 9.30 and Matlab software, the characteristic peaks of UPLC fingerprint of safflower from different varieties and different habitats in Xinjiang were analyzed by cluster analysis and principal component analysis. Results the specific UPLC fingerprint of Carthamus sativus L. was established and 26 common peaks were established. The quality of 30 batches of Xinjiang safflower samples and 4 batches of Henan safflower samples had great difference, and there were some differences among different regions of Xinjiang and different varieties from the same area. Cluster analysis and principal component analysis (PCA) were used to identify the fingerprint differences of Xinjiang safflower. Conclusion the method is simple, effective and reliable. It provides an effective and rapid evaluation method for the quality control of Carthamus tinctorius.
【作者单位】：北京中医药大学中药学院中药品质评价北京市重点实验室;福建医科大学药学院天然药物学系;北京联馨药业有限公司;
【分类号】：R284.1;O657.72

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