自制纳米粒子载体结合无标记适体传感器化学发光检测应用

发布时间：2018-10-22 17:42

【摘要】：食品中污染物的含量影响着人类健康与生命安全,对其进行直接或者间接的监测是十分必要的。为了研究操作方便、灵敏可靠、快速的检测技术,本文制备两种纳米粒子,设计基于自制纳米粒子的无标记适体传感器并优化检测条件,以化学发光法检测三磷酸腺苷(Adenosine triphosphate,ATP)、氯霉素、赭曲霉毒素A(Ochratoxin A,OTA)这三种与食品安全息息相关的物质。主要内容如下:(1)基于自制Fe_3O_4纳米粒子的无标记适体传感器用于检测ATP首先利用共沉淀法制备出Fe_3O_4纳米粒子,随后利用X射线衍射、傅里叶变换红外光谱、磁性、紫外可见吸收光谱及透射电镜等对其组成及粒径进行表征,最后以该粒子为载体制备无标记适体传感器,对ATP进行化学发光法检测;结果表明,该自制Fe_3O_4粒子的粒径均一、磁性强、稳定性好、氨基改性效果良好,ATP的检测限为6.80×10-7 mol/L,并应用于检测酸奶样品中的ATP,检测得每毫升酸奶中ATP含量为3.98×10-8 moL;(2)基于自制SiO_2纳米粒子的无标记适体传感器用于检测氯霉素首先利用溶胶-凝胶法制备出SiO_2纳米粒子,随后分别以商品化磁性微球和自制SiO_2纳米粒子为载体制备无标记适体传感器用于检测氯霉素;结果表明基于商品化磁性微球、自制SiO_2纳米粒子的无标记适体传感器对氯霉素的检测限分别为3.57×10-9 mol/L、3.26×10-9 mol/L,并应用于检测加标牛奶样品中的氯霉素,检出率在94.1%~103.0%之间;(3)基于自制Fe_3O_4纳米粒子和自制SiO_2纳米粒子的无标记适体传感器用于检测双组份中ATP和OTA通过磁性分离,基于自制SiO_2纳米粒子的无标记适体传感器用于检测ATP,基于自制Fe_3O_4纳米粒子的无标记适体传感器用于检测OTA;结果表明,ATP和OTA的检测限分别为1.43×10-7 mol/L和8.32×10-8 mol/L,并应用于加标啤酒样品中ATP和OTA的检测,对ATP和OTA的检出率在94.2%~101.0%之间。
[Abstract]:The content of contaminants in food affects human health and life safety, so it is necessary to monitor it directly or indirectly. In order to study the convenient, sensitive, reliable and rapid detection technology, two kinds of nanoparticles were prepared in this paper. The unlabeled aptamer sensor based on self-made nanoparticles was designed and the detection conditions were optimized. Adenosine triphosphate,ATP, chloramphenicol and ochratoxin A (Ochratoxin were detected by chemiluminescence method, which were closely related to food safety. The main contents are as follows: (1) the unlabeled aptamer sensor based on self-made Fe_3O_4 nanoparticles was used to detect ATP. Firstly, Fe_3O_4 nanoparticles were prepared by coprecipitation method, then X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), magnetic properties were used. The composition and particle size were characterized by UV-Vis absorption spectrum and transmission electron microscope. Finally, the unlabeled aptamer sensor was prepared with the particle as the carrier, and the ATP was detected by chemiluminescence method. The results showed that the diameter of the self-made Fe_3O_4 particle was uniform. Strong magnetism, good stability, The ATP detection limit was 6.80 脳 10 ~ (-7) mol/L, and the content of ATP was 3.98 脳 10 ~ (-8) moL; per milliliter of yoghurt. (2) the unlabeled aptamer sensor based on self-made SiO_2 nanoparticles was used to detect chloramphenicol. SiO_2 nanoparticles were prepared by sol-gel method. Subsequently, unlabeled aptamer sensors were prepared on commercial magnetic microspheres and home-made SiO_2 nanoparticles as carriers for the detection of chloramphenicol, the results showed that commercial magnetic microspheres were based on commercial magnetic microspheres. The detection limit of chloramphenicol with self-made SiO_2 nanoparticles was 3.57 脳 10 ~ (-9) mol/L,3.26 脳 10 ~ (-9) mol/L, and was applied to the detection of chloramphenicol in milk samples. The detection rate was between 94.1% and 103.0%. (3) unlabeled aptamer sensors based on home-made Fe_3O_4 nanoparticles and home-made SiO_2 nanoparticles were used to detect the magnetic separation of ATP and OTA in two components. Unlabeled aptamer sensor based on homemade SiO_2 nanoparticles for detection of ATP, unlabeled aptamer sensor based on home-made Fe_3O_4 nanoparticles for OTA; detection the detection limits of ATP and OTA are 1.43 脳 10-7 mol/L and 8.32 脳 10-8 mol/L, respectively. Determination of ATP and OTA in standard beer samples, The detection rate of ATP and OTA was between 94.2% and 101.0%.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;TS207.5

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