HPLC-ELSD法测定三种植物基原金线莲的金线莲苷含量
发布时间：2018-10-25 20:06
【摘要】：建立HPLC-ELSD法,测定三种植物基原金线莲中金线莲苷含量。采用phenomenex NH2色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(92∶8,V/V)为流动相进行等度洗脱,流速为0.8 m L/min,柱温室温,ELSD雾化室温度为70℃,氮气流速为1.5 L/min。实验结果表明,金线莲苷进样量在76.02~2432.64μg/m L范围内线性良好(r=0.9991);精密度、稳定性、重复性实验的RSD均≤3%;平均加样回收率为97.55%,RSD为1.50%(n=6)。33批次金线莲的金线莲苷含量在6.37%~22.66%之间,平均含量为15.00%,存在较大的差异(RSD=26.29%)。该方法快速、简便,结果准确、可靠,适用于金线莲中金线莲苷的定量分析。
[Abstract]:A HPLC-ELSD method was established for the determination of the content of goldenine in three plant species. Phenomenex NH2 column (250 mm 脳 4.6 mm,5 渭 m),) with acetonitrile-water (92: 8 V / V) as mobile phase was used for iso-elution at a flow rate of 0.8 mL / min, column temperature in greenhouse, and temperature of ELSD atomization chamber at 70 鈩，
本文编号：2294704
[Abstract]:A HPLC-ELSD method was established for the determination of the content of goldenine in three plant species. Phenomenex NH2 column (250 mm 脳 4.6 mm,5 渭 m),) with acetonitrile-water (92: 8 V / V) as mobile phase was used for iso-elution at a flow rate of 0.8 mL / min, column temperature in greenhouse, and temperature of ELSD atomization chamber at 70 鈩，
本文编号：2294704
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