分散固相萃取-液相色谱-串联质谱法测定典型小宗作物中6种农药残留

发布时间：2018-11-01 20:00

【摘要】：建立生姜、红薯、土豆、荔枝中多菌灵、吡虫啉、涕灭威、三唑酮、乙草胺、苯醚甲环唑的液相色谱-串联质谱检测方法。样品经乙腈溶液提取,50 mg N-丙基乙二胺吸附剂、25 mg十八烷基键合硅胶(C_(18))净化,C_(18)色谱柱分离,0.1%甲酸溶液-甲醇作为流动相进行梯度洗脱,在电喷雾正离子电离模式下,质谱多离子监测模式定量分析,基质标准曲线外标法定量。结果表明,6种农药在10~500μg/L范围内呈良好的线性关系,相关系数(r)均大于0.995。3个加标水平下,6种农药的平均回收率为87.9%~110.3%,相对标准偏差不大于14.6%。该方法简单、快速、灵敏度高,能够满足小宗作物农药残留的监控要求。
[Abstract]:A method for the determination of carbendazim, imidacloprid, aldicarb, triazolone, Acetochlor and diphenyl ether triacyclozole in ginger, sweet potato, potato and litchi was established by liquid chromatography-tandem mass spectrometry (LC-MS). The samples were extracted by acetonitrile solution, 50 mg N-propyl ethylenediamine adsorbent, 25 mg octadecyl bonded silica gel (C18) purified, separated by C18 column, and then eluted with 0.1% formic acid solution and methanol as the mobile phase. In the electrospray ion ionization mode, mass spectrometry multi-ion monitoring mode quantitative analysis, matrix standard curve external standard curve quantitative analysis. The results showed that there was a good linear relationship in the range of 10 渭 g / L for 6 pesticides, and the correlation coefficient (r) was greater than 0.995.3. The average recovery of the six pesticides was 87.9% and 110.3%, respectively. The relative standard deviation is not greater than 14.6. The method is simple, rapid and sensitive, and can meet the monitoring requirements of pesticide residues in small crops.
【作者单位】：中国农业科学院农业质量标准与检测技术研究所农业部农产品质量安全重点实验室;
【基金】：国家自然科学基金面上项目(31471654) “十二五”国家科技支撑计划项目(2014BAD13B05-05) 中央级公益性科研院所基本科研业务费专项(1610072006009)
【分类号】：O657.63;TQ450.263

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