高乌头炮制品HPLC指纹图谱建立及质量研究

发布时间：2018-11-06 13:24

【摘要】：目的建立统一条件的色谱指纹图谱鉴别和含量测定分析方法,用于不同产区高乌头炮制品的质量研究。方法通过HPLC梯度洗脱建立高乌头炮制品的指纹图谱,并采用中药指纹图谱相似度评价系统(2012版)、主成分分析(PCA)和聚类分析(CA)对指纹图谱进行分析。结果建立了高乌头炮制品指纹图谱,10批高乌头制品的相似度均大于0.90,标定共有峰18个,并对其中2主要成分(高乌甲素、冉乌头碱)进行含量测定,聚类分析(CA)将所有批次高乌头制品分为4类,反映了10批不同产区高乌头制品的质量特征;主成分分析筛选出累计贡献率达到88.824%的4个主成分,得到决定高乌头药材质量7个化学成分。结论该方法重现性好、特征性强可用于高乌头炮制品全面质量评价。
[Abstract]:Objective to establish a method of chromatographic fingerprint identification and determination and analysis for the quality of processed products of Aconitum aconitum in different production areas. Methods HPLC gradient elution was used to establish the fingerprint of processed product of Aconitum aconitum. The fingerprint was analyzed by (PCA) and cluster analysis (CA) with the similarity evaluation system of traditional Chinese medicine fingerprint (version 2012). Results the fingerprint of processed aconitum was established. The similarity of 10 batches of high aconitum products was greater than 0.90, 18 peaks were calibrated, and the contents of 2 main components (aconitine, ranaconitine) were determined. Cluster analysis (CA) divided all batches of high aconitum products into 4 categories, which reflected the quality characteristics of 10 batches of high aconitum products in different production areas. Four principal components with a cumulative contribution rate of 88.824% were screened by principal component analysis, and 7 chemical constituents were obtained to determine the quality of high aconitum. Conclusion the method has good reproducibility and can be used to evaluate the total quality of processed aconitum.
【作者单位】：甘肃中医药大学;甘肃省中藏药化学与质量研究省级重点实验室;
【基金】：国家自然科学基金资助项目(81560650) 甘肃省自然基金资助项目(1107RJZA242)
【分类号】：O657.72;R284.1

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