基于DNA折纸模板的金纳米颗粒自组装及其等离子体共振效应研究
发布时间：2018-11-06 12:46
【摘要】：纳米金属颗粒具有高电子密度、介电特性和催化作用,以及优异的生物相容性等优势,在催化、传感器和生物医学等方面展现出巨大的应用潜力。利用“自下而上”的方法在纳米级精度上有序的组装贵金属纳米颗粒是纳米技术研究的重要方向。DNA“折纸术”是将天然的DNA长链进行反复折叠,并用短链加以固定,由此获得一系列形状各异的二维、三维DNA结构。在DNA折纸结构中,每条单链的位置都是独特的,因此其具有纳米级高度可寻址性,为定位组装金属纳米颗粒提供了良好的模板。DNA纳米技术为纳米颗粒的精确定位,调控颗粒间相互作用和有效控制纳米颗粒自组装提供了更加丰富的途径。本文主要利用DNA折纸结构的多样性和可寻址性,将金属纳米颗粒构建成不同的纳米结构,并对其法诺性质进行研究。基于上述内容,本论文的研究内容主要分为以下三个方面:1.利用晶种生长法分别制备了形貌均一,尺寸可控的纳米金棒和纳米金立方。为了实现实验所需求的纳米金棒尺寸,通过调节晶种和硝酸银的加入量,可以获得不同长径比的纳米金棒;同样,通过调节晶种的加入量,获得目标尺寸和形貌均一的纳米金立方。金属纳米颗粒的制备,为后续实验提供充足的原材料和为研究其光学性质提供有力的保障。2.利用DNA折纸的纳米级可寻址性来定位组装纳米金棒,形成类似多尔门型的纳米金等离子体结构。通过对纳米金棒DNA全修饰,利用DNA折纸上定位伸出的捕获链与修饰在纳米金棒表面的DNA杂交,将金纳米棒组装到DNA折纸上精密加工成多尔门构型。采用暗场显微镜与扫描电子显微镜共定位的方法对形成的多尔门型等离子体结构研究法诺共振效应。3.进一步探索以DNA折纸为模板的纳米金等离子体结构在法诺共振效应的研究。以三角形DNA折纸为模板,精密加工纳米金立方和纳米金棒形成棒方二聚体,并通过多种方法表征样品的结构特征。我们采取暗场显微镜与扫描电子显微镜共定位的方法对形成的不同构型的棒方二聚体的散射光谱进行采集,并研究纳米金二聚体的等离子体性质。
[Abstract]:Nanometallic particles have many advantages such as high electron density, dielectric properties and catalytic properties, and excellent biocompatibility, showing great potential in catalysis, biosensor and biomedicine. Using "bottom-up" method to assemble noble metal nanoparticles in nanoscale order is an important direction of nanotechnology research. DNA origami is to fold natural DNA long chains repeatedly and fix them with short chains. A series of 2D and 3D DNA structures with different shapes are obtained. In the DNA origami structure, the position of each single strand is unique, so it is highly addressable at nanometer level, which provides a good template for locating and assembling metal nanoparticles. Regulation of particle interaction and effective control of nanoparticles self-assembly provide a more abundant approach. In this paper, the diversity and addressable properties of DNA origami structure were used to construct metal nanoparticles into different nanostructures, and the properties of Fano were studied. Based on the above content, the research content of this paper is divided into the following three aspects: 1. Nanocrystalline gold bars and nanocrystalline gold cubes with uniform morphology and controllable size were prepared by seed growth method. In order to realize the size of nanocrystalline gold rod, the nanocrystalline gold rod with different aspect ratio can be obtained by adjusting the amount of crystal seed and silver nitrate. Similarly, the nanocrystalline gold cubes with uniform target size and morphology were obtained by adjusting the amount of seed added. The preparation of metal nanoparticles provides sufficient raw materials for subsequent experiments and provides a strong guarantee for the study of their optical properties. 2. Nano-level addressable properties of DNA origami paper were used to locate and assemble nanocrystalline gold rods to form a door-like nanocrystalline gold plasma structure. The gold nanorods were fabricated on DNA origami by fully modifying the gold nanorods with DNA, and the trapping chains located and extended on the DNA origami were hybridized with the DNA modified on the surface of the nanorods, and the gold nanorods were assembled into DNA origami. The Farno resonance effect was studied by means of colocation of dark field microscope and scanning electron microscope. Further study on the resonance effect of nanocrystalline gold plasma with DNA origami as template was carried out. Using triangular DNA origami as template, nanocrystalline gold cubes and nanocrystalline gold rods were fabricated to form rod-square dimers, and the structural characteristics of the samples were characterized by various methods. The scattering spectra of nanocrystalline gold dimers with different configurations were collected by co-localization of dark field microscope and scanning electron microscope and the plasma properties of nanocrystalline gold dimers were studied.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O614.123
本文编号：2314321
[Abstract]:Nanometallic particles have many advantages such as high electron density, dielectric properties and catalytic properties, and excellent biocompatibility, showing great potential in catalysis, biosensor and biomedicine. Using "bottom-up" method to assemble noble metal nanoparticles in nanoscale order is an important direction of nanotechnology research. DNA origami is to fold natural DNA long chains repeatedly and fix them with short chains. A series of 2D and 3D DNA structures with different shapes are obtained. In the DNA origami structure, the position of each single strand is unique, so it is highly addressable at nanometer level, which provides a good template for locating and assembling metal nanoparticles. Regulation of particle interaction and effective control of nanoparticles self-assembly provide a more abundant approach. In this paper, the diversity and addressable properties of DNA origami structure were used to construct metal nanoparticles into different nanostructures, and the properties of Fano were studied. Based on the above content, the research content of this paper is divided into the following three aspects: 1. Nanocrystalline gold bars and nanocrystalline gold cubes with uniform morphology and controllable size were prepared by seed growth method. In order to realize the size of nanocrystalline gold rod, the nanocrystalline gold rod with different aspect ratio can be obtained by adjusting the amount of crystal seed and silver nitrate. Similarly, the nanocrystalline gold cubes with uniform target size and morphology were obtained by adjusting the amount of seed added. The preparation of metal nanoparticles provides sufficient raw materials for subsequent experiments and provides a strong guarantee for the study of their optical properties. 2. Nano-level addressable properties of DNA origami paper were used to locate and assemble nanocrystalline gold rods to form a door-like nanocrystalline gold plasma structure. The gold nanorods were fabricated on DNA origami by fully modifying the gold nanorods with DNA, and the trapping chains located and extended on the DNA origami were hybridized with the DNA modified on the surface of the nanorods, and the gold nanorods were assembled into DNA origami. The Farno resonance effect was studied by means of colocation of dark field microscope and scanning electron microscope. Further study on the resonance effect of nanocrystalline gold plasma with DNA origami as template was carried out. Using triangular DNA origami as template, nanocrystalline gold cubes and nanocrystalline gold rods were fabricated to form rod-square dimers, and the structural characteristics of the samples were characterized by various methods. The scattering spectra of nanocrystalline gold dimers with different configurations were collected by co-localization of dark field microscope and scanning electron microscope and the plasma properties of nanocrystalline gold dimers were studied.
【学位授予单位】：南京邮电大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TB383.1;O614.123
【参考文献】
相关期刊论文 前2条
1 杨洋;柳华杰;刘冬生;;DNA纳米机器[J];化学进展;2008年Z1期
2 ;Analogic China map constructed by DNA[J];Chinese Science Bulletin;2006年24期
，本文编号：2314321
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