RP-HPLC法测定复方消银颗粒中没食子酸、黄芩苷和黄芩素的含量

发布时间：2018-11-07 16:33

【摘要】：目的:建立RP-HPLC法同时测定复方消银颗粒中没食子酸、黄芩苷和黄芩素3个化学成分含量。方法:采用Shim-pack ODS(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱(0~22 min,3%A→10%A;22~35 min,10%A→35%A;35~50 min,35%A→28%A),流速1.0 m L·min~(-1),检测波长为280 nm,柱温35℃。结果:没食子酸、黄芩苷和黄芩素质量浓度分别在3.57~49.98μg·m L~(-1)(r=0.999 5)、4.032~40.32μg·m L~(-1)(r=0.999 4)和3.278~16.390μg·m L~(-1)(r=0.999 3)范围内呈良好线性关系,平均回收率在98.74%~100.1%范围内,RSD在0.49%~1.5%范围内;3批样品中没食子酸、黄芩苷和黄芩素含量分别为0.153~0.154、0.256~0.258、0.113~0.114 mg·g~(-1)。结论:本方法可为复方消银颗粒多指标质量评价及控制提供科学依据。
[Abstract]:Objective: to establish a RP-HPLC method for the simultaneous determination of Gallic acid, baicalin and baicalin in compound Xiaoyin granules. Methods: Shim-pack ODS (250 mm 脳 4. 6 mm,5 渭 m) column was used with acetonitrile (A)-0.1% phosphoric acid solution (B) as mobile phase. Gradient elution was used. The flow rate was 1.0 mL min~ (-1). The detection wavelength was 280 nm, column temperature 35 鈩，

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