薄层层析-图像分析定量热凝胶β-1,3-葡寡糖

发布时间：2018-11-10 18:30

【摘要】：单向两次薄层层析展开可以显著提高对热凝胶β-1,3-葡寡糖的分析效果,以Silica gel60 F254硅胶板为固定相,V(乙酸)∶V(乙酸乙酯)∶V(水)=2∶2.4∶1.4为展开剂,展距20 cm时,单向两次展开可以较好地分离聚合度为2~13的热凝胶β-1,3-葡寡糖。此外还建立了Image J软件图像分析定量热凝胶β-1,3-葡寡糖的方法,在不同糖质量浓度范围(0.02~0.1、0.1~0.8、0.8~5.0 g/L)内寡糖显色斑点的灰度积分响应值与糖浓度分别有着很好的线性关系(R20.99)。该方法的灵敏度高达0.02 g/L,相对标准偏差为1%~5%,稳定性很好。与HPLC等方法相比具有操作简便、分析快速、不需专门仪器和特定寡糖标准品等优点。
[Abstract]:Unidirectional double TLC could improve the analytical effect of thermogel 尾 -1N 3-glucooligosaccharides. The Silica gel60 F254 silica gel plate was used as the stationary phase, V (): V (ethyl acetate): V (water) = 2: 2. 4: 1.4 as the developing agent. When the span is 20 cm, the thermal gel 尾 -1C _ 3-glucooligosaccharides with polymerization degree of 2o _ (13) can be separated by unidirectional twice-unwrapping. In addition, a Image J image analysis method for quantitative thermal gel 尾 -1C 3- glucooligosaccharides was established. There was a good linear relationship between the gray integral response value of oligosaccharide color spots and sugar concentration (R20.99) in different sugar concentration range (0.02 ~ 0.1g / L). The sensitivity of the method is as high as 0.02 g / L, the relative standard deviation is 1 / 5 and the stability is very good. Compared with HPLC and other methods, the method is simple, rapid, and does not require special instruments and specific oligosaccharide standard.
【作者单位】：江南大学生物工程学院/糖化学与生物技术教育部重点实验室;江苏瑞光生物科技有限公司;
【基金】：国家自然科学基金项目(31171640,31201384,31271888) 无锡市科技支撑计划项目(CLE01N1208) 无锡市生物农业领军型创业人才计划项目(“130”计划) 无锡中小企业创新基金项目(CBE01G1344)
【分类号】：TQ427.26;O658.1

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