HPLC法测定甜叶菊中6个酚酸类成分含量

发布时间：2018-11-10 23:14

【摘要】：目的:建立高效液相色谱法同时测定甜叶菊叶中新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A和异绿原酸C6个酚酸类成分。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),流动相为0.1%甲酸水溶液-乙腈,梯度洗脱,柱温30℃,检测波长327 nm。结果:6个酚酸质量浓度在10~196μg·m L-1范围内线性关系良好;6个酚酸的加样回收率分别为98.3%、95.2%、100.1%、97.3%、95.6%和96.1%,加样回收率、精密度、重复性、稳定性均符合有关规定;9批样品中6个酚酸类成分的含量分别为新绿原酸0.062%~0.725%,绿原酸0.277%~2.450%,隐绿原酸0.096%~0.694%,异绿原酸B 0.046%~0.867%,异绿原酸A0.157%~0.772%,异绿原酸C 0.135%~1.821%,6个酚酸之和在1.182%~6.668%。结论:该方法可为甜叶菊进一步开发利用提供质量控制方法。
[Abstract]:Objective: to establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of 6 phenolic acids in stevia leaves such as Neo-Lv Yuan Acid, Cryptom_person1# Acid, Isobalipironic Acid B, IsoLv Yuan Acid A and isoLv Yuan Acid C6. Methods: C18 column (4.6 mm 脳 250 mm,5 渭 m),) was used as mobile phase of 0.1% formic acid-acetonitrile, gradient elution, column temperature 30 鈩，

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