肉桂不同部位及其挥发油的红外光谱宏观表征

发布时间：2018-11-15 21:45

【摘要】：目的:考察肉桂不同部位(树皮、嫩枝、叶)及其挥发油的红外指纹图谱差异,为研究其不同部位所含化学成分特征提供参考依据。方法:采用傅里叶变换红外光谱法及二阶导数红外光谱法。结果:肉桂的树皮、嫩枝、叶原粉末红外光谱整体峰形较相似,三者均含有草酸钙、多酚类及糖苷类成分;嫩枝和叶在1 653 cm-1和1 734 cm~(-1)附近的特征峰要明显强于皮,推测嫩枝和叶中饱和脂肪酸酯类成分和黄酮类成分的含量要高于皮。采用二阶导数放大后,发现在1 480~1 435 cm~(-1)和1 630~1 580 cm~(-1)时,嫩枝有2个特征峰,而叶在相应位置只有1个特征峰。树皮、嫩枝、叶挥发油红外光谱与桂皮醛的光谱相近,通过相关系数的计算,发现三者挥发油中桂皮醛含量大小依次为树皮嫩枝叶;在1 734 cm~(-1)处附近,嫩枝和叶挥发油的峰强要明显大于树皮,说明嫩枝和叶的挥发油中还含有其他结构的酯类化合物。通过比较二阶导数光谱中1 275 cm~(-1)处峰附近的高低,可以进一步区分嫩枝和叶的挥发油。结论:红外光谱及二阶导数红外光谱法,不仅可以分析肉桂不同部位及其挥发油的整体化学成分差异,还可以区分含有相似成分的不同部位。
[Abstract]:Objective: to study the IR fingerprint of different parts of Cinnamomum cinnamon (bark, twigs, leaves) and its volatile oil, and to provide a reference for studying the chemical constituents of different parts of cinnamon. Methods: Fourier transform infrared spectroscopy and second derivative infrared spectroscopy were used. Results: the IR spectra of bark, twigs and leaves of Cinnamomum cinnamon were similar in shape. All of them contained calcium oxalate, polyphenols and glycosides. The characteristic peaks of shoots and leaves near 1 653 cm-1 and 1 734 cm~ (-1) were significantly stronger than those of bark, and the contents of saturated fatty acid esters and flavonoids in shoots and leaves were estimated to be higher than those of bark. After amplification with second derivative, it was found that at 1 480 cm~ (-1) and 1 630 ~ 1 580 cm~ (-1), the twigs had two characteristic peaks, while the leaves had only one characteristic peak at the corresponding position. The infrared spectrum of the essential oil of bark, twig and leaf was similar to that of cinnamaldehyde. By the calculation of correlation coefficient, it was found that the content of cinnamaldehyde in the volatile oil was in order of bark, twig and leaf. In the vicinity of 1 734 cm~ (-1), the peak strength of the volatile oil of twigs and leaves was significantly higher than that of bark, indicating that the volatile oils of twigs and leaves also contained esters with other structures. By comparing the levels near the peak of 1 275 cm~ (-1) in the second derivative spectrum, the volatile oils of twigs and leaves can be further distinguished. Conclusion: infrared spectrum and second derivative infrared spectroscopy can not only analyze the difference of chemical composition of different parts of Cinnamomum cinnamon and its volatile oil, but also distinguish different parts of cinnamon with similar components.
【作者单位】：北京中医药大学中药学院;淄博万杰中医药研究所;
【基金】：国家自然科学基金项目(81303217,81374027)
【分类号】：R284.1;O657.33

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