基因载体材料聚乙烯亚胺与磷脂酰胆碱脂质体的相互作用对膜结构的影响

发布时间：2018-11-16 07:19

【摘要】：采用动态光散射、荧光光谱、zeta电位测定和等温滴定量热技术分析了分子量分别为25000,10000和1800的聚乙烯亚胺(PEI)与二油酰磷脂酰胆碱(DOPC)脂质体的相互作用及其对脂质体膜内环境极性和膜通透性的影响.结果表明,PEI通过氨基与DOPC的磷脂基团和胆碱基团产生氢键或范德华作用,从而与脂质体结合形成复合物;低浓度PEI(0.075 mg/mL)导致DOPC脂质体的聚集和表面电位的增加,但未引起脂质体膜融合和表面电位反转;进一步增加PEI的浓度对脂质体表面电位的影响很小,而结合在表面的PEI分子链之间的排斥作用阻碍了脂质体聚集.PEI分子与DOPC脂质体的结合降低了脂质分子碳氢链的堆积密度和脂质体膜内环境的疏水性,从而增强了钙黄绿素和槲皮素在脂质体膜中的通透性.PEI与DOPC脂质体的相互作用具有明显的分子尺寸效应,增大PEI的分子量可以增强与脂质体的相互作用及对脂质体膜结构的影响.
[Abstract]:Dynamic light scattering, fluorescence spectrum, The interaction of polyethyleneimide (PEI) with dioleoylphosphatidylcholine (DOPC) liposomes with molecular weight of 25000,000,000,000,1800 and their effects on the environmental polarity and membrane permeability of liposomes were analyzed by zeta potentiometry and isothermal drop calorimetry. The results showed that PEI formed a complex with liposomes by hydrogen bonding or van der Waals interaction between amino groups and phospholipid and choline groups of DOPC. Low concentration of PEI (0.075 mg/mL) induced the aggregation and surface potential of DOPC liposomes, but did not cause membrane fusion and surface potential reversal. Further increasing the concentration of PEI had little effect on the surface potential of liposomes. However, the repulsive effect between PEI chains on the surface hindered the aggregation of liposomes. The binding of PEI molecules to DOPC liposomes reduced the density of hydrocarbon chains and the hydrophobicity of liposomes. Therefore, the permeability of calcitonin and quercetin in liposome membranes was enhanced. The interaction between PEI and DOPC liposomes had a significant molecular size effect. Increasing the molecular weight of PEI can enhance the interaction with liposomes and influence the structure of liposome membranes.
【作者单位】：广西大学化学化工学院;
【基金】：国家自然科学基金(批准号:21373062)资助~~
【分类号】：O641.3

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