基于RRLC-DAD-ESI-Q-TOF-MS技术的山东金银花多指标定量指纹图谱分析
发布时间：2018-11-28 20:48
【摘要】：目的:运用RRLC-DAD-ESI-Q-TOF-MS技术分析和测定金银花中主要化学成分,建立山东金银花多指标定量指纹图谱。方法:采用RRLC-DAD-ESI-Q-TOF-MS技术对金银花提取物进行快速分析,根据获得的精确相对分子质量和二级质谱碎片信息推断化合物分子式,结合数据库和文献对金银花中化学成分进行鉴别,采用RRLC-DAD法分析测定山东金银花、河南金银花和湖南山银花中主要化学成分含量,在此基础上建立了山东金银花多指标定量指纹图谱。结果:从山东金银花中检测到51种化学成分,鉴定了其中32种化学成分;对比测定了山东金银花、河南金银花和湖南山银花中10种主要化学成分(马钱酸、绿原酸、咖啡酸、马钱苷、断氧化马钱苷、芦丁、金桃苷、木犀草苷、异绿原酸A和异绿原酸C)的含量;采用指纹图谱结合相似度分析可以实现不同品种金银花和湖南山银花的正确区分,所得结果与含量测定结果一致。结论:RRLC-DAD-ESIQ-TOF-MS技术分析速度快、灵敏度高、能够提供化合物及其碎片离子的精确质量信息,可以快速准确地鉴别和测定金银花中的化学成分。在此基础上建立的山东道地金银花的多指标定量指纹图谱,为山东金银花质量评价研究提供了技术支持。
[Abstract]:Objective: to analyze and determine the main chemical constituents in Lonicera japonica by RRLC-DAD-ESI-Q-TOF-MS, and to establish quantitative fingerprint of Flos Lonicerae in Shandong Province. Methods: the extract of Flos Lonicerae was analyzed quickly by RRLC-DAD-ESI-Q-TOF-MS, and the molecular formula of the compound was deduced according to the accurate molecular weight and the information of second order mass spectrometry. The chemical constituents of Flos Lonicerae in Shandong Province, Henan Province and Hunan Province were determined by RRLC-DAD method. On this basis, the quantitative fingerprint of honeysuckle in Shandong was established. Results: 51 chemical constituents were detected from Flos Lonicerae in Shandong Province and 32 of them were identified. Ten main chemical constituents (equinic acid, Lv Yuan acid, caffeic acid, riganoside, Rutin, hyperin, luteolin) in Honeysuckle, Henan Flos Lonicerae and Honeysuckle in Hunan Province were determined, and the chemical constituents of Flos Lonicerae, Honeysuckle, Honeysuckle and Honeysuckle in Hunan were determined. Contents of isoLv Yuan acid A and isob_person1# acid C; Fingerprint and similarity analysis can be used to distinguish different varieties of Flos Lonicerae from Honeysuckle in Hunan Province. The results are consistent with the results of content determination. Conclusion: RRLC-DAD-ESIQ-TOF-MS can provide accurate mass information of compounds and their fragments, and can be used to identify and determine the chemical constituents of Flos Lonicerae. On this basis, the quantitative fingerprint of Lonicera japonica in Shandong Province was established, which provided technical support for the quality evaluation of Lonicera japonica in Shandong.
【作者单位】： 山东省中药质量控制技术重点实验室山东省分析测试中心;山东中医药大学药学院;山东农业大学食品科学与工程学院;济南三峰生物工程责任有限公司;
【基金】：国家自然科学基金项目(811300700,81473298) 山东省科技发展计划项目(2014GSF119031,2016GSF202014) 中央本级重大增减支项目(2060302) 山东省自然基金项目(ZR2013HM075)
【分类号】：O657.63;R284.1
本文编号：2364216
[Abstract]:Objective: to analyze and determine the main chemical constituents in Lonicera japonica by RRLC-DAD-ESI-Q-TOF-MS, and to establish quantitative fingerprint of Flos Lonicerae in Shandong Province. Methods: the extract of Flos Lonicerae was analyzed quickly by RRLC-DAD-ESI-Q-TOF-MS, and the molecular formula of the compound was deduced according to the accurate molecular weight and the information of second order mass spectrometry. The chemical constituents of Flos Lonicerae in Shandong Province, Henan Province and Hunan Province were determined by RRLC-DAD method. On this basis, the quantitative fingerprint of honeysuckle in Shandong was established. Results: 51 chemical constituents were detected from Flos Lonicerae in Shandong Province and 32 of them were identified. Ten main chemical constituents (equinic acid, Lv Yuan acid, caffeic acid, riganoside, Rutin, hyperin, luteolin) in Honeysuckle, Henan Flos Lonicerae and Honeysuckle in Hunan Province were determined, and the chemical constituents of Flos Lonicerae, Honeysuckle, Honeysuckle and Honeysuckle in Hunan were determined. Contents of isoLv Yuan acid A and isob_person1# acid C; Fingerprint and similarity analysis can be used to distinguish different varieties of Flos Lonicerae from Honeysuckle in Hunan Province. The results are consistent with the results of content determination. Conclusion: RRLC-DAD-ESIQ-TOF-MS can provide accurate mass information of compounds and their fragments, and can be used to identify and determine the chemical constituents of Flos Lonicerae. On this basis, the quantitative fingerprint of Lonicera japonica in Shandong Province was established, which provided technical support for the quality evaluation of Lonicera japonica in Shandong.
【作者单位】： 山东省中药质量控制技术重点实验室山东省分析测试中心;山东中医药大学药学院;山东农业大学食品科学与工程学院;济南三峰生物工程责任有限公司;
【基金】：国家自然科学基金项目(811300700,81473298) 山东省科技发展计划项目(2014GSF119031,2016GSF202014) 中央本级重大增减支项目(2060302) 山东省自然基金项目(ZR2013HM075)
【分类号】：O657.63;R284.1
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