HPLC法同时测定钩吻茎中胡蔓藤碱丙等6个生物碱的含量

发布时间：2018-11-28 19:48

【摘要】：目的:建立HPLC法同时测定钩吻茎中胡蔓藤碱丙、钩吻素甲、钩吻素子、钩吻素己、钩吻绿碱和胡蔓藤碱乙的含量,并比较评价各产地钩吻生物碱含量的差异。方法:采用外标法进行测定,色谱柱为ZORBAX Bonus-RP Analytical(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%甲酸水梯度洗脱,流速1.0 m L·min~(-1),检测波长254 nm,柱温30℃。分别测定32批钩吻茎,并进行聚类分析。结果:胡蔓藤碱丙、钩吻素甲、钩吻素子、钩吻素己、钩吻绿碱、胡蔓藤碱乙6个成分的线性范围分别为1.00~50.06μg·mL~(-1)(r=1.000 0)、1.13~50.85μg·mL~(-1)(r=0.999 8)、1.16~52.20μg·mL~(-1)(r=1.000 0)、1.16~52.20μg·mL~(-1)(r=0.999 7)、1.06~50.88μg·mL~(-1)(r=0.999 7)和1.01~50.50μg·mL~(-1)(r=1.000 0),平均加样回收率为93.1%~103.8%(RSD为2.0%~2.9%)。不同产地样品中钩吻茎中胡蔓藤碱丙、钩吻素甲、钩吻素子、钩吻素己、钩吻绿碱和胡蔓藤碱乙含量范围分别为0.136~2.202、0.564~2.686、0.718~3.553、0.160~2.025、0.151~3.493、0.288~4.700 mg·g~(-1)。结果显示不同产地的钩吻6个成分含量均有差异,32批不同产地钩吻药材可聚为6大类。结论:该法可为钩吻多指标成分的质量评价提供参考。不同产地钩吻的各个化学成分的含量呈现明显分类特点,为钩吻地区鉴定提供依据。
[Abstract]:Objective: to establish a HPLC method for the simultaneous determination of alkaloids of Gelsemine C, Geptosin A, Gelsemin, Gelsemine and Echinine B in the stems of Gelsemium amurensis, and to compare and evaluate the differences of the contents of the alkaloids of Gelsemine in different areas. Methods: the external standard method was used. The chromatographic column was ZORBAX Bonus-RP Analytical (4.6 mm 脳 250 mm,5 渭 m),) mobile phase was methanol-0.1% formic acid water gradient elution, the flow rate was 1.0 mL min~ (-1), the detection wavelength was 254 nm, column temperature 30 鈩，

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